Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4081-4086. doi: 10.1099/ijs.0.050567-0. Epub 2013 May 31.
Two novel isolates of rapidly growing, Gram-stain-positive, non-chromogenic species of the genus Mycobacterium, strain YIM M13028(T) from a sediment sample collected from the South China Sea (19° 30.261' N 111° 0.247' E) at a depth of 42 m and strain YIM 121001(T) from a coastal zone sand sample collected in Dubai, United Arab Emirates, were obtained in our laboratory. Their taxonomic positions were determined by a polyphasic approach. Good growth of the two strains was observed at 28 °C and pH 7.0 with 0-2 % NaCl on tryptic soy agar medium. Both strains formed round orange-red colonies, strain YIM M13028(T) had a rough surface, while YIM 121001(T) was smooth. Cellular fatty acids, whole-cell protein profiles and TLC analysis of their mycolic acids show significant differences from reference stains. Phenotypic characteristics and multilocus sequence analysis (MLSA) of 16S rRNA gene, hsp65, rpoB and 16S-23S internal transcribed spacer (ITS) sequences indicated that both strains YIM M13028(T) and YIM 121001(T) belong to the genus Mycobacterium. DNA-DNA hybridization values revealed a low relatedness (<70 %) of the two isolates with the type strains Mycobacterium neoaurum DSM 44074(T) and Mycobacterium hodleri DSM 44183(T). The low DNA-DNA hybridization values (40.4±3.5 %) between strains YIM M13028(T) and YIM 121001(T) and phenotypic distinctiveness indicated that the two strains were representatives of different novel species of the genus Mycobacterium. The names proposed for these novel species are Mycobacterium sediminis sp. nov. and Mycobacterium arabiense sp. nov., and the type strains are YIM M13028(T) ( = DSM 45643(T) = KCTC 19999(T)) and YIM 121001(T) ( = DSM 45768(T) = JCM 18538(T)), respectively.
从南海（北纬 19°30.261'，东经 111°0.247'，深度 42 米）沉积物样本和阿拉伯联合酋长国迪拜沿海地区沙样本中，我们实验室分别获得了两株新型、快速生长、革兰氏阳性、非产色分枝杆菌属的分离株，菌株 YIM M13028(T)和 YIM 121001(T)。通过多相分类法确定了它们的分类地位。这两株菌在 28°C 和 pH 为 7.0 时，在含 0-2%NaCl 的胰蛋白胨大豆琼脂培养基中生长良好。两株菌均形成圆形橙红色菌落，菌株 YIM M13028(T)表面粗糙，而 YIM 121001(T)表面光滑。细胞脂肪酸、全细胞蛋白图谱和分枝菌酸 TLC 分析结果与参考菌株存在显著差异。16S rRNA 基因、hsp65、rpoB 和 16S-23S 内转录间隔区（ITS）序列的多位点序列分析（MLSA）以及表型特征表明，两株 YIM M13028(T)和 YIM 121001(T)菌株均属于分枝杆菌属。DNA-DNA 杂交值显示，这两株分离株与模式种嗜麦芽寡养单胞菌 DSM 44074(T)和海分枝杆菌 DSM 44183(T)的亲缘关系较低（<70%）。菌株 YIM M13028(T)和 YIM 121001(T)之间的低 DNA-DNA 杂交值（40.4±3.5%）和表型差异表明，这两株菌是分枝杆菌属不同新型种的代表。为这些新型种命名为海洋分枝杆菌（Mycobacterium sediminis）sp. nov.和阿拉伯分枝杆菌（Mycobacterium arabiense）sp. nov.，其模式株分别为 YIM M13028(T)（=DSM 45643(T)=KCTC 19999(T)）和 YIM 121001(T)（=DSM 45768(T)=JCM 18538(T)）。
