Li Mali, Ma Yanping, Ji Yaohua, He Rong, Qi Ying, Sun Zhengrong, Wang Ning, Gao Shuang, Ruan Qiang
China Medical University, Shenyang, Liaoning, People's Republic of China.
Virus Genes. 2011 Dec;43(3):327-34. doi: 10.1007/s11262-011-0641-5. Epub 2011 Jul 13.
Human cytomegalovirus (HCMV) RL13 gene is a member of the RL11 gene family. To study the expression kinetics and transcript structures of the gene, screening of cDNA library, Northern blot, 3' and 5' RACE analyses were performed with a low-passaged clinical strain. The results showed that the RL13 gene was mainly transcribed in the late expression phase with at least seven forms of transcripts of 3628, 3114, 2515-2443, 1737, 1240, 1029 and 846 nt, respectively. All these transcripts were unspliced with an identical 3' terminal and the same typical polyA signal "AATAAA". Except for the transcript of 846 nt, RL13 open reading frames (ORFs) were 909 bp and completely identical in all of the transcripts. The sequence of the RL13 ORF in the examined clinical strain had a higher similarity with that of the UL153 ORF in the Towne strain than those of RL13 ORF in any other HCMV strains.
人类巨细胞病毒（HCMV）RL13基因是RL11基因家族的成员。为了研究该基因的表达动力学和转录本结构，使用低传代临床毒株进行了cDNA文库筛选、Northern印迹、3'和5' RACE分析。结果表明，RL13基因主要在晚期表达阶段转录，分别有至少7种形式的转录本，长度分别为3628、3114、2515 - 2443、1737、1240、1029和846 nt。所有这些转录本均未剪接，具有相同的3'末端和相同的典型多聚腺苷酸信号“AATAAA”。除了846 nt的转录本外，RL13开放阅读框（ORF）为909 bp，且在所有转录本中完全相同。在所检测的临床毒株中，RL13 ORF的序列与Towne毒株中UL153 ORF的序列相似性高于任何其他HCMV毒株中RL13 ORF的序列。
