Department of Anatomy, School of Veterinary Medicine, University of California Davis, Davis, California 95616, USA.
J Cell Biochem. 2011 Nov;112(11):3354-63. doi: 10.1002/jcb.23265.
Tenascins regulate cell interaction with the surrounding pericellular matrix. Within bone, tenascins C and W influence osteoblast adhesion and differentiation, although little is known about the regulation of tenascin expression. In this study we examined the effect of osteogenic differentiation, bone morphogenetic protein (BMP) and Wnt growth factors, and mechanical loading on tenascin expression in osteogenic cells. Osteogenic differentiation increased tenascin C (TnC), and decreased tenascin W (TnW), expression. Both growth factors and mechanical loading increased both TnC and TnW expression, albeit via distinct signaling mechanisms. Both BMP-2 and Wnt5a induction of tenascin expression were mediated by MAP kinases. These data establish a role for BMP, Wnts, and mechanical loading in the regulation of tenascin expression in osteoblasts.
纤连蛋白调节细胞与周围细胞外基质的相互作用。在骨骼中，纤连蛋白 C 和 W 影响成骨细胞的黏附和分化，尽管关于纤连蛋白表达的调控知之甚少。在这项研究中，我们研究了成骨分化、骨形态发生蛋白 (BMP) 和 Wnt 生长因子以及机械加载对成骨细胞中纤连蛋白表达的影响。成骨分化增加了纤连蛋白 C (TnC)，而降低了纤连蛋白 W (TnW) 的表达。两种生长因子和机械加载都增加了 TnC 和 TnW 的表达，尽管它们通过不同的信号机制。BMP-2 和 Wnt5a 诱导纤连蛋白表达都是通过 MAP 激酶介导的。这些数据确立了 BMP、Wnt 和机械加载在调节成骨细胞中纤连蛋白表达中的作用。
