Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093, USA.
J Nat Prod. 2011 Aug 26;74(8):1737-43. doi: 10.1021/np200236c. Epub 2011 Jul 13.
The evolutionary relationships of cyanobacteria, as inferred by their SSU (16S) rRNA genes, were used as predictors of their potential to produce varied secondary metabolites. The evolutionary relatedness in geographically distant cyanobacterial specimens was then used as a guide for the detection and isolation of new variations of predicted molecules. This phylogeny-guided isolation approach for new secondary metabolites was tested in its capacity to direct the search for specific classes of new natural products from Curaçao marine cyanobacteria. As a result, we discovered ethyl tumonoate A (1), a new tumonoic acid derivative with anti-inflammatory activity and inhibitory activity of calcium oscillations in neocortical neurons.
根据蓝藻的 SSU（16S）rRNA 基因推断其进化关系，可作为其产生不同次生代谢产物的潜力的预测因子。然后，利用地理上遥远的蓝藻标本的进化相关性来指导预测分子的新变体的检测和分离。这种基于系统发育的新型次生代谢产物分离方法用于指导从库拉索海洋蓝藻中寻找特定类别的新型天然产物。结果，我们发现了乙基 Tumonoate A（1），这是一种新型的 Tumonoic 酸衍生物，具有抗炎活性和新皮质神经元钙震荡的抑制活性。
