Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093-0204, USA.
J Nat Prod. 2011 Aug 26;74(8):1773-8. doi: 10.1021/np200323e. Epub 2011 Jul 13.
Three polyenylpyrone metabolites, pyridinopyrones A to C (1-3), have been isolated from the culture broth of a marine-derived Streptomyces sp., strain CNQ-301. The structures of the pyridinopyrones were assigned on the basis of chemical modification and combined spectroscopic methods, focusing on interpretation of 1D and 2D NMR data. Pyridinopyrones B and C (2, 3), examined as an inseparable mixture of methyl positional isomers, were ultimately defined by hydrogenation and NMR analysis of a saturated derivative. The biosynthesis of these metabolites was defined by the incorporation of stable isotope-labeled precursors, revealing that the biosynthetic starter unit is nicotinic acid, while the polyene chain and pendant methyl groups are acetate- and methionine-derived, respectively.
从海洋来源的链霉菌 CNQ-301 的发酵液中分离到三种聚烯基吡啶酮代谢物，吡啶酮 A 到 C（1-3）。基于化学修饰和综合光谱方法，特别是对 1D 和 2D NMR 数据的解释，确定了吡啶酮的结构。作为甲基位置异构体混合物不可分离的混合物，吡啶酮 B 和 C（2、3）最终通过饱和衍生物的氢化和 NMR 分析来定义。这些代谢物的生物合成通过掺入稳定同位素标记的前体来定义，表明生物合成的起始单元是烟酸，而多烯链和侧挂甲基分别来自乙酸盐和蛋氨酸。
