Pharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
Org Lett. 2012 Oct 5;14(19):5050-3. doi: 10.1021/ol3022758. Epub 2012 Sep 17.
Drug resistant infectious diseases are quickly becoming a global health crisis. While Streptomyces spp. have been a major source of antibiotics over the past 50 years, efficient methods are needed to identify new antibiotics and greatly improve the rate of discovery. LCMS-based metabolomics were applied to analyze extracts of 50 Streptomyes spp. Using this methodology, we discovered bottromycin D and used whole genome sequencing to determine its biosynthesis by a ribosomal pathway.
耐药性传染病迅速成为全球健康危机。虽然链霉菌属在过去 50 年中是抗生素的主要来源，但仍需要有效的方法来识别新的抗生素并极大地提高发现的速度。基于 LCMS 的代谢组学被应用于分析 50 株链霉菌属的提取物。使用这种方法，我们发现了 bottromycin D，并通过全基因组测序确定了其通过核糖体途径合成。
