Gupta Shallu, Sharma Tarun Kumar, Kaushik G G, Shekhawat V P S
Department of Biochemistry, J.L.N. Medical College, Ajmer, Rajasthan, India.
Clin Lab. 2011;57(5-6):379-86.
Increasing evidence in both experimental and clinical studies suggests that free radical mediated oxidative stress plays a major role in the pathogenesis of both types of diabetes mellitus. Proteins and lipids are among the prime targets for oxidative stress. In this study we evaluated oxidative stress in Type 1 Diabetes Mellitus (insulin dependent diabetes mellitus, IDDM) patients by estimating lipid peroxidation and the effect of vitamin E on oxidative stress and metabolic parameters.
A total of 40 children (20 Type 1 Diabetes Mellitus patients + 20 healthy controls) were examined in the study. Oxidative stress parameters malondialdehyde (MDA), antioxidants, reduced glutathione (GSH), vitamin E and metabolic parameters were studied. All the type 1 Diabetes Mellitus patients were supplemented with 600 mg/daily vitamin E for three months. After three months of supplementation all the parameters mentioned above were studied again.
Reduced glutathione and vitamin E levels were lower and malondialdehyde levels were higher in Type 1 Diabetes Mellitus patients compared to healthy controls (p < 0.05). After supplementation with vitamin E in diabetic patients a significant decrease (p < 0.05) in MDA levels and significant increase in GSH (p < 0.05) and vitamin E (p < 0.05) levels were found. A negative correlation between MDA and vitamin E, between MDA and GSH and a positive correlation between vitamin E and GSH was found. Significant changes were not observed in metabolic parameters in Type 1 Diabetes Mellitus patients after vitamin E supplementation (p > 0.05).
Vitamin E ameliorates oxidative stress in Type 1 Diabetes Mellitus patients and improves antioxidant defense system. However, vitamin E does not have any advantage for metabolic parameters.
实验研究和临床研究中越来越多的证据表明，自由基介导的氧化应激在两种类型的糖尿病发病机制中起主要作用。蛋白质和脂质是氧化应激的主要靶点。在本研究中，我们通过评估脂质过氧化以及维生素E对氧化应激和代谢参数的影响，来评估1型糖尿病（胰岛素依赖型糖尿病，IDDM）患者的氧化应激情况。
本研究共检查了40名儿童（20名1型糖尿病患者 + 20名健康对照）。研究了氧化应激参数丙二醛（MDA）、抗氧化剂、还原型谷胱甘肽（GSH）、维生素E以及代谢参数。所有1型糖尿病患者每日补充600毫克维生素E，为期三个月。补充三个月后，再次研究上述所有参数。
与健康对照相比，1型糖尿病患者的还原型谷胱甘肽和维生素E水平较低，丙二醛水平较高（p < ）。糖尿病患者补充维生素E后，MDA水平显著降低（p < ），GSH（p < ）和维生素E（p < ）水平显著升高。发现MDA与维生素E之间、MDA与GSH之间呈负相关，维生素E与GSH之间呈正相关。1型糖尿病患者补充维生素E后，代谢参数未观察到显著变化（p > ）。
维生素E可改善1型糖尿病患者的氧化应激，提高抗氧化防御系统。然而，维生素E对代谢参数没有任何益处。
