Department of Respiratory Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
Department of Respiratory Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
Chest. 2012 Jan;141(1):176-182. doi: 10.1378/chest.11-0068. Epub 2011 Jul 14.
Mucosa-associated lymphoid tissue (MALT) lymphoma constitutes approximately 90% of primary pulmonary lymphoma, and the diagnosis of pulmonary MALT lymphoma often requires invasive methods such as surgical lung biopsy. Chromosomal rearrangements involving MALT lymphoma translocation gene 1 (MALT1) have been reported to be specific for MALT lymphoma. The combination of BAL and cytologic approaches with molecular methods is useful for the diagnosis of lymphoproliferative disorders. Therefore, we examined the detection of MALT1 gene rearrangements in BAL fluid (BALF) cells for the diagnosis of MALT lymphoma.
We determined the percentage of BALF cells with MALT1 gene rearrangements by using the fluorescence in situ hybridization (FISH) method in 10 patients suspected to have pulmonary MALT lymphoma.
MALT1 gene rearrangements in BALF cells were found in four of five cases with pulmonary MALT lymphoma (percentage of BALF cells with MALT1 gene rearrangements: 21.8% ± 6.8%). On the other hand, MALT1 gene rearrangements in BALF cells were negative in the five cases without pulmonary MALT lymphoma and one case with pulmonary MALT lymphoma.
These results suggest that the detection of MALT1 gene rearrangements in BALF cells is useful for the diagnosis of pulmonary MALT lymphoma, as it is a specific method that is less invasive than surgical biopsy. Because of the small number of patients in this study, further investigations are necessary to evaluate the detection rate of MALT1 gene rearrangements in BALF cells from patients with pulmonary MALT lymphoma.
黏膜相关淋巴组织（MALT）淋巴瘤约占原发性肺淋巴瘤的 90%，肺 MALT 淋巴瘤的诊断通常需要采用侵袭性方法，如外科肺活检。已报道涉及 MALT 淋巴瘤易位基因 1（MALT1）的染色体重排对于 MALT 淋巴瘤是特异性的。BAL 和细胞学方法与分子方法的结合对于淋巴增生性疾病的诊断是有用的。因此，我们研究了 BAL 液（BALF）细胞中 MALT1 基因重排的检测在 MALT 淋巴瘤诊断中的作用。
我们通过荧光原位杂交（FISH）方法检测了 10 例疑似患有肺 MALT 淋巴瘤患者的 BALF 细胞中 MALT1 基因重排的百分比。
在五例肺 MALT 淋巴瘤中，有四例 BALF 细胞中存在 MALT1 基因重排（BALF 细胞中存在 MALT1 基因重排的百分比：21.8%±6.8%）。另一方面，在五例无肺 MALT 淋巴瘤和一例肺 MALT 淋巴瘤患者的 BALF 细胞中，MALT1 基因重排均为阴性。
这些结果表明，BALF 细胞中 MALT1 基因重排的检测对于肺 MALT 淋巴瘤的诊断是有用的，因为它是一种比外科活检侵袭性更小的特异性方法。由于本研究中的患者数量较少，因此需要进一步研究来评估肺 MALT 淋巴瘤患者 BALF 细胞中 MALT1 基因重排的检测率。
