Department of Pathology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
Am J Clin Pathol. 2011 Aug;136(2):252-9. doi: 10.1309/AJCPDXFOPXGEF0RP.
Counting mitotic figures (MFs) is one of the essential factors for determining the histologic grade of pulmonary neuroendocrine carcinoma (NEC). We analyzed MFs by using a mitotic-specific antibody of phosphohistone H3 (PHH3) in 113 lung NECs (66 typical carcinoids [TCs], 12 atypical carcinoids [ACs], 20 large cell NECs [LCNECs], and 15 small cell lung carcinomas [SCLCs]). Subdivided by histologic subtype, the mean PHH3-stained MFs (mPHMFs) were 0.09 per high-power field (hpf) in TCs, 0.39/hpf in ACs, 7.84/hpf in LCNECs, and 9.42 in SCLCs. From the 5-year overall survival rate for mPHMFs, an mPHMF of more than 1.0 was the best threshold in all NECs and an mPHMF of more than 0.4 was the best threshold for differentiating ACs from TCs. These values correspond to 4/10 hpf and 10/10 hpf. We showed that the PHH3-based mitosis-counting method is a reliable, easy method for counting mitoses in pulmonary NECs.
计数有丝分裂象（MFs）是确定肺神经内分泌癌（NEC）组织学分级的重要因素之一。我们使用磷酸化组蛋白 H3（PHH3）的有丝分裂特异性抗体分析了 113 例肺 NEC（66 例典型类癌[TCs]，12 例非典型类癌[ACs]，20 例大细胞 NEC [LCNECs]和 15 例小细胞肺癌[SCLCs]）中的 MFs。按组织学亚型细分，TCs 中 PHH3 染色的平均 MFs（mPHMFs）为 0.09 个/高倍视野（hpf），ACs 为 0.39/hpf，LCNECs 为 7.84/hpf，SCLCs 为 9.42。从 mPHMFs 的 5 年总生存率来看，mPHMF 超过 1.0 是所有 NEC 的最佳阈值，mPHMF 超过 0.4 是区分 ACs 和 TCs 的最佳阈值。这些值相当于 4/10 hpf 和 10/10 hpf。我们表明，基于 PHH3 的有丝分裂计数方法是一种可靠、简便的方法，可用于计数肺 NEC 中的有丝分裂。
