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SIF结合元件赋予c-fos启动子对sis/PDGF的诱导性。

The SIF binding element confers sis/PDGF inducibility onto the c-fos promoter.

作者信息

Wagner B J, Hayes T E, Hoban C J, Cochran B H

机构信息

MIT Center for Cancer Research, Cambridge.

出版信息

EMBO J. 1990 Dec;9(13):4477-84. doi: 10.1002/j.1460-2075.1990.tb07898.x.

DOI:10.1002/j.1460-2075.1990.tb07898.x
PMID:2176154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC552240/
Abstract

The c-fos proto-oncogene is rapidly and transiently induced by a variety of extracellular stimuli. We have previously shown that conditioned media from v-sis transformed NRK cells rapidly induces a DNA binding protein which binds to a conserved sequence upstream of the human c-fos gene. We now show that purified recombinant c-sis/PDGF can induce this binding activity which we have termed SIF, for sis-inducible factor. Oligonucleotides which bind to the SIF protein will confer sis/PDGF inducibility onto a truncated, unresponsive c-fos promoter. However, sequences lying between -100 and -57 of the c-fos gene are required for this induction. The sis-responsive element functions independently of a region of dyad symmetry previously identified as the serum responsive element (SRE). The time course of c-fos expression driven by the sis-responsive element is similar to that mediated by the SRE. Unlike the SRE, which can respond to signals generated by sis/PDGF, serum or phorbol esters, the SIF binding element mediates c-fos induction only in response to sis/PDGF. The SRE and SIF elements function in an additive manner to stimulate the transcription of the c-fos gene in response to sis/PDGF.

摘要

原癌基因c-fos可被多种细胞外刺激快速、短暂地诱导。我们先前已表明，来自v-sis转化的NRK细胞的条件培养基能快速诱导一种DNA结合蛋白，该蛋白可与人c-fos基因上游的保守序列结合。我们现在发现，纯化的重组c-sis/血小板衍生生长因子（PDGF）能诱导这种结合活性，我们将其称为SIF，即sis诱导因子。与SIF蛋白结合的寡核苷酸能赋予截短的、无反应性的c-fos启动子sis/PDGF诱导性。然而，c-fos基因-100至-57之间的序列是这种诱导所必需的。sis反应元件的功能独立于先前确定为血清反应元件（SRE）的二元对称区域。由sis反应元件驱动的c-fos表达的时间进程与由SRE介导的相似。与可对sis/PDGF、血清或佛波酯产生的信号作出反应的SRE不同，SIF结合元件仅在对sis/PDGF作出反应时介导c-fos诱导。SRE和SIF元件以相加的方式发挥作用，以响应sis/PDGF刺激c-fos基因的转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/ef94e8314d6f/emboj00240-0270-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/000c60fb3da5/emboj00240-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/2cc25e91f463/emboj00240-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/9701c19a0286/emboj00240-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/3779e3db0e26/emboj00240-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/ef94e8314d6f/emboj00240-0270-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/000c60fb3da5/emboj00240-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/2cc25e91f463/emboj00240-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/9701c19a0286/emboj00240-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/3779e3db0e26/emboj00240-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a95/552240/ef94e8314d6f/emboj00240-0270-b.jpg

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