Nanomedicine and Nanobiotechnology Laboratory, University of South Florida, 4202 East Fowler Avenue, Tampa, Florida 33620, United States.
ACS Appl Mater Interfaces. 2011 Aug;3(8):2994-3001. doi: 10.1021/am200498r. Epub 2011 Jul 26.
Fiber mats of a synthetic anionic copolypeptide of l-glutamic acid and l-tyrosine (PLEY) have been produced by electrospinning, and physical, chemical, and biological properties of the fibers have been characterized in vitro. Fibers were obtained from polymer dissolved in water at concentrations of 20-60% (w/v) but not below this range. Applied voltage and spinneret-collector distance were also found to influence polymer spinnability. Oriented fibers were obtained by changing the geometry of the collector. Fiber diameter was measured by scanning electron microscopy (SEM). A common chemical reagent was used to cross-link polymers postspinning. Fiber solubility in aqueous solution varied as a function of cross-linking time. Cationic polypeptides labeled with a fluorescent dye became noncovalently associated with cross-linked fibers, enabling visualization by fluorescence microscopy. Spectroscopy provided information on polymer chain conformation in solution and in fibers. Degradation of cross-linked fibers by different proteases has been demonstrated. Fibroblasts were cultured on cross-linked fiber mats to test basic cytocompatibility. Synthetic polypeptide fiber mats may be useful in applications in medicine, biotechnology, and other areas.
已通过静电纺丝生产出合成阴离子共多肽聚谷氨酸和 L-酪氨酸（PLEY）的纤维垫，并对纤维的物理、化学和生物学特性进行了体外表征。纤维是由浓度为 20-60%（w/v）的聚合物在水中溶解而获得的，但不能低于此范围。施加的电压和喷丝头-收集器之间的距离也被发现会影响聚合物的可纺性。通过改变收集器的几何形状可以获得取向纤维。纤维直径通过扫描电子显微镜（SEM）进行测量。使用一种常见的化学试剂在纺丝后对聚合物进行交联。纤维在水溶液中的溶解度随交联时间而变化。用荧光染料标记的阳离子多肽与交联纤维非共价结合，通过荧光显微镜可以观察到。光谱学提供了聚合物链在溶液和纤维中的构象信息。已经证明不同蛋白酶对交联纤维的降解作用。已经在交联纤维垫上培养了成纤维细胞，以测试基本的细胞相容性。合成多肽纤维垫在医学、生物技术和其他领域的应用可能是有用的。
