Asian Natural Environmental Science Center (ANESC), The University of Tokyo, 1-1-1 Midori-cho, Nishitokyo-shi, Tokyo, 188-0002, Japan.
Plant Methods. 2011 Jul 15;7:22. doi: 10.1186/1746-4811-7-22.
Protein extraction is a frequent procedure in biological research. For preparation of plant cell extracts, plant materials usually have to be ground and homogenized to physically break the robust cell wall, but this step is laborious and time-consuming when a large number of samples are handled at once.
We developed a chemical method for lysing Arabidopsis cells without grinding. In this method, plants are boiled for just 10 minutes in a solution containing a Ca2+ chelator and detergent. Cell extracts prepared by this method were suitable for SDS-PAGE and immunoblot analysis. This method was also applicable to genomic DNA extraction for PCR analysis. Our method was applied to many other plant species, and worked well for some of them.
Our method is rapid and economical, and allows many samples to be prepared simultaneously for protein analysis. Our method is useful not only for Arabidopsis research but also research on certain other species.
蛋白质提取是生物研究中的常见步骤。对于植物细胞提取物的制备，通常需要将植物材料研磨和匀浆以物理破坏坚固的细胞壁，但当一次处理大量样品时，这个步骤既费力又耗时。
我们开发了一种无需研磨即可裂解拟南芥细胞的化学方法。在该方法中，将植物在含有 Ca2+螯合剂和去污剂的溶液中煮沸仅 10 分钟。通过该方法制备的细胞提取物适用于 SDS-PAGE 和免疫印迹分析。该方法也适用于用于 PCR 分析的基因组 DNA 提取。我们的方法适用于许多其他植物物种，并且对其中一些物种有效。
我们的方法快速且经济实惠，可同时为蛋白质分析准备多个样本。我们的方法不仅对拟南芥研究有用，对某些其他物种的研究也有用。
