Khojasteh Arash, Behnia Hossein, Dashti Seyedeh Ghazaleh, Stevens Mark
Division of Basic Sciences, Dental Research Center, Department of Oral and Maxillofacial Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Oral Maxillofac Surg. 2012 Apr;70(4):972-82. doi: 10.1016/j.joms.2011.02.133. Epub 2011 Jul 16.
The literature regarding mesenchymal stem cell (MSC)-based bone reconstruction techniques are sparse and no comprehensive review of current methods has been performed. The aim of this article was to provide a discussion of clinical and experimental reports of MSC application in the reconstruction of bony defects in live models.
This search was executed using the PubMed database with various combinations of related keywords. Currently published English-language studies that had applied MSCs as a part of their treatment protocol for reconstruction of bony defects in rat, rabbit, dog, and human models were reviewed. The included studies had reported substantiation that the applied cells were of MSC origin as a part of the study design. Publications inclusive to February 1, 2010 were evaluated. Of review of 187 found abstracts and full texts, 25 articles met the inclusion criteria.
Based on this review, tremendous differences exist among investigators for the application of MSCs in bone augmentation procedures. These differences include not only species uniqueness but also a plethora of other variances, such as stem cell source, defect sites and sizes, carriers and constructs, use of additional growth factors, measured parameters, and methods of data collection.
Because of the multitude of protocols, range of parameters, and data in the current English-language literature, this review did not reach any significant conclusion as to the "most predictable" model in stem cell reconstruction. However, it does "shed light" on the need for additional collaborated studies using similar homogenous designs and data analysis in advancing the science of bone reconstruction using MSCs.
关于基于间充质干细胞（MSC）的骨重建技术的文献稀少，且尚未对当前方法进行全面综述。本文旨在探讨在活体模型中MSC应用于骨缺损重建的临床和实验报告。
使用PubMed数据库，通过相关关键词的各种组合进行检索。对目前已发表的将MSC作为大鼠、兔、犬和人类模型骨缺损重建治疗方案一部分的英文研究进行综述。纳入的研究报告了在研究设计中已证实所应用的细胞来源于MSC。对截至2010年2月1日的出版物进行评估。在对187篇找到的摘要和全文进行审查后，25篇文章符合纳入标准。
基于本次综述，研究人员在MSC应用于骨增量手术方面存在巨大差异。这些差异不仅包括物种特异性，还包括许多其他差异，如干细胞来源、缺损部位和大小、载体和构建物、是否使用额外的生长因子、测量参数以及数据收集方法。
由于当前英文文献中有众多方案、参数范围和数据，本次综述未就干细胞重建中“最可预测”的模型得出任何重要结论。然而，它确实“揭示”了在推进使用MSC进行骨重建科学方面，需要开展更多采用相似同质设计和数据分析的合作研究。
