利用样本身份的数字编码进行大规模转座元件插入位点的绘制。
Large-scale mapping of transposable element insertion sites using digital encoding of sample identity.
机构信息
Department of Neurobiology, Stanford University, Stanford, California 94305.
出版信息
Genetics. 2014 Mar;196(3):615-23. doi: 10.1534/genetics.113.159483. Epub 2013 Dec 27.
Abstract
Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections of transposon insertions, individualized amplification and sequencing is both time consuming and costly. We describe an approach in which large numbers of insertion lines can be simultaneously mapped in a single DNA sequencing reaction by using digital error-correcting codes to encode line identity in a unique set of barcoded pools.
摘要
确定转座元件的基因组位置是一个常见的实验目标。当绘制大量转座子插入的图谱时，单独的扩增和测序既耗时又昂贵。我们描述了一种方法，通过使用数字纠错码将线的身份编码在一组独特的带条码的池中来在单个 DNA 测序反应中同时映射大量的插入线。
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