Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Nan-Tzu, Kaohsiung City 811, Taiwan.
Arch Microbiol. 2012 Feb;194(2):103-12. doi: 10.1007/s00203-011-0730-9. Epub 2011 Jul 16.
A bacterial strain designated antisso-27(T), previously isolated from saltpan in Taiwan while screening for bacteria for algicidal activity, was characterized using the polyphasic taxonomic approach. Strain antisso-27(T) was Gram-negative, aerobic, brownish yellow colored, rod-shaped, non-flagellated and non-gliding. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain antisso-27(T) belonged to the genus Aquimarina within the family Flavobacteriaceae with relatively low sequence similarities of 94.0-96.6% to other valid Aquimarina spp. It contained iso-C(17:0) 3-OH, iso-C(15:0), iso-C(16:0), iso-C(15:1) and iso-C(15:0) 3-OH as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone. Major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, an uncharacterized aminolipid and five uncharacterized phospholipids. Strain antisso-27(T) employed direct mode of algicidal lysis to Chlorella vulgaris strain 211-31; nevertheless, it released an algicidal substance against M. aeruginosa strain MTY01. This is the first study that the Aquimarina species possesses both direct and indirect algicidal activities. On the basis of the phylogenetic and phenotypic data, strain antisso-27(T) should be classified as representing a novel species, for which the name A. salinaria sp. nov. is proposed. The type strain is A. salinaria antisso-27(T) (= BCRC 80080(T) = LMG 25375(T)).
一株编号为 antisso-27(T) 的细菌菌株，之前在台湾盐田筛选具有杀藻活性的细菌时被分离出来，使用多相分类方法进行了特征描述。菌株 antisso-27(T) 为革兰氏阴性、需氧、棕黄色、杆状、无鞭毛和滑行。基于 16S rRNA 基因序列的系统发育分析表明，菌株 antisso-27(T) 属于黄杆菌科的 Aquimarina 属，与其他有效 Aquimarina spp. 的序列相似性相对较低，为 94.0-96.6%。它含有 iso-C(17:0) 3-OH、iso-C(15:0)、iso-C(16:0)、iso-C(15:1) 和 iso-C(15:0) 3-OH 作为主要脂肪酸，并含有六异戊二烯单位的menaquinone (MK-6) 作为主要类异戊二烯醌。主要极性脂为磷脂酰乙醇胺、双磷脂酰甘油、一种未鉴定的胺脂和五种未鉴定的磷脂。菌株 antisso-27(T) 采用直接溶藻的方式杀死小球藻 211-31 菌株；然而，它释放出一种杀藻物质来对抗铜绿微囊藻 MTY01 菌株。这是首次研究表明 Aquimarina 物种具有直接和间接的杀藻活性。根据系统发育和表型数据，菌株 antisso-27(T) 应归类为代表一个新种，建议将其命名为 A. salinaria sp. nov.。模式菌株为 A. salinaria antisso-27(T) (=BCRC 80080(T)=LMG 25375(T))。
