García Dabeiba A, Cid-Arregui Angel, Schmitt Markus, Castillo Marcos, Briceño Ignacio, Aristizábal Fabio A
Centro de Investigaciones Odontológicas, Pontificia Universidad Javeriana, Bogota, Colombia.
Open Virol J. 2011;5:70-9. doi: 10.2174/1874357901105010070. Epub 2011 Jun 17.
Cancer of the uterine cervix (CC) is the second most common cancer in women worldwide. In Colombia, CC is the second most frequent cancer among the entire women population and the first among women aged between 15 and 44 years, with an estimated incidence of 24.9 cases/100,000 inhabitants. The main risk factor is infection with one or more high-risk human papillomavirus (HPV) types. The aim of this study was to estimate the genotype-specific prevalence of human papillomavirus (HPV) DNA in patients with cervical pathology using the multiplex PCR and Luminex xMAP technology. In addition, we compared genotyping with Luminex xMAP and with Reverse Line Blot (RLB). A cohort of 160 patients participated in the study, of which 25.6% had no cervical lesions, 35% presented cervical intraepithelial neoplasia of grade I (CIN I), 10% CIN II, 20.6% CIN III and 8.8% CC. The most frequent viral types in all lesion grades were HPV16 and HPV18. Infections by a unique virus were less frequent (19.4%) than multiple infections (80.6%). Single infections were found in 22% of women with no cervical lesions, and in 14.3% of CIN I, 18.7% CIN II, 21.2% CIN III and 28.6% of CC. Multiple infections were observed in 78.0% of cervical samples with negative histopathologic diagnosis, and in 85.7% of CIN I, 81.2% CIN II, 78.8% CIN III and 71.4% CC. All samples analyzed with Luminex xMAP were HPV-positive, while we could detect HPV in only 48.8% of cases with RLB. Of the samples positive by both methods, there was a 67.2% correlation in the viral type(s) detected. In conclusion, Luminex suspension array showed a remarkably higher sensitivity compared with RLB. Multiple infections were unexpectedly common, being HPV types 16 and 18 the most prevalent in all histopathologic grades.
子宫颈癌（CC）是全球女性中第二常见的癌症。在哥伦比亚，CC是全体女性人群中第二常见的癌症，在15至44岁女性中则是最常见的癌症，估计发病率为每10万居民中有24.9例。主要风险因素是感染一种或多种高危型人乳头瘤病毒（HPV）。本研究的目的是使用多重PCR和Luminex xMAP技术估计宫颈病变患者中人乳头瘤病毒（HPV）DNA的基因型特异性流行率。此外，我们将Luminex xMAP基因分型与反向线印迹法（RLB）进行了比较。160名患者参与了该研究，其中25.6%没有宫颈病变，35%表现为一级宫颈上皮内瘤变（CIN I），10%为CIN II，20.6%为CIN III，8.8%为CC。在所有病变级别中最常见的病毒类型是HPV16和HPV18。单一病毒感染的频率（19.4%）低于多重感染（80.6%）。在没有宫颈病变的女性中，22%发现单一感染，在CIN I中为14.3%，CIN II中为18.7%，CIN III中为21.2%，CC中为28.6%。在组织病理学诊断为阴性的宫颈样本中，78.0%观察到多重感染，在CIN I中为85.7%，CIN II中为81.2%，CIN III中为78.8%，CC中为71.4%。所有用Luminex xMAP分析的样本均为HPV阳性，而用RLB仅能在48.8%的病例中检测到HPV。在两种方法均为阳性的样本中，检测到的病毒类型有67.2%的相关性。总之，与RLB相比，Luminex悬浮阵列显示出显著更高的灵敏度。多重感染出乎意料地常见，HPV16和HPV18在所有组织病理学级别中最为普遍。
