Schmitt F, Cochand-Priollet B, Toetsch M, Davidson B, Bondi A, Vielh P
Medical Faculty of Porto, University and Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal.
Cytopathology. 2011 Aug;22(4):238-42. doi: 10.1111/j.1365-2303.2011.00885.x.
This report describes the results of the first chosen topic of the European Federation of Cytology Societies (EFCS) scientific committee, which concerns the application of immunocytochemistry (ICC) to cytological material. While ICC has become an important ancillary method, not only for diagnosis but also to assess prognostic and predictive factors on cytological material, there are many different methodologies used and the lack of standardization has been criticized. This inquiry aimed first to obtain an overview of the techniques used and second to suggest mechanisms for standardization and quality control.
We report the results of 28 replies from 13 countries to a web-based inquiry into types of specimens, preparations and technical methods.
Conventional smears were the preparations most commonly used, followed by cytospins, cell blocks and liquid-based preparations, in that order. Avidin-biotin complex, labelled streptavidin-biotin or peroxidase anti-peroxidase were used in 61% and enhanced-polymer technology in 39%. Automated staining techniques were used by 64%. More than half used the same antibody dilutions as histopathology although only 20% used cell blocks.
Reduction of variability by using automation, appropriate controls and customized dilution of antibodies according to different samples could improve the quality of ICC and standardize the techniques, along with quality control and quality assurance measures.
本报告描述了欧洲细胞学会联合会（EFCS）科学委员会首个选定主题的结果，该主题涉及免疫细胞化学（ICC）在细胞学材料中的应用。虽然ICC不仅已成为诊断的重要辅助方法，而且还用于评估细胞学材料的预后和预测因素，但使用的方法多种多样，缺乏标准化受到了批评。这项调查的目的首先是概述所使用的技术，其次是提出标准化和质量控制的机制。
我们报告了来自13个国家的28份回复的结果，这些回复是针对一项基于网络的关于标本类型、制备方法和技术方法的调查。
传统涂片是最常用的制备方法，其次依次是细胞离心涂片、细胞块和液基制片。61%的人使用抗生物素蛋白-生物素复合物、标记链霉抗生物素蛋白-生物素或过氧化物酶抗过氧化物酶，39%的人使用增强聚合物技术。64%的人使用自动染色技术。超过一半的人使用与组织病理学相同的抗体稀释度，尽管只有20%的人使用细胞块。
通过使用自动化、适当的对照以及根据不同样本定制抗体稀释度来减少变异性，同时采取质量控制和质量保证措施，可以提高ICC的质量并使技术标准化。
