Institute of Biology, Laboratory of Eco-Epidemiology of Parasites, University of Neuchâtel, Emile Argand 11, Case postale 158, 2009 Neuchâtel, Switzerland.
Ticks Tick Borne Dis. 2010 Mar;1(1):23-9. doi: 10.1016/j.ttbdis.2009.11.001. Epub 2009 Dec 16.
In Europe, 7 Borrelia species belonging to the Borrelia burgdorferi sensu lato complex have been reported in Ixodes ricinus ticks. In addition, another Borrelia, related to the relapsing fever spirochaetes, has also been described. In the present study, we designed probes for reverse line blotting allowing detection and identification of all these Borrelia species after amplification of the variable spacer region between the 23S and 5S ribosomal genes. These new probes allowed us investigate the diversity of Borrelia in 915 I. ricinus collected on the south-facing slope of Chaumont (Switzerland). Among the 159 infected ticks, 7 Borrelia species were identified, and B. spielmanii and relapsing fever-like (RFL) spirochaetes were identified in this area for the first time. B. valaisiana and B. spielmanii were significantly less present in male than in female or nymphal ticks. Mixed infection with RFL spirochaetes and Lyme borreliosis spirochaetes were detected in 4 ticks. In addition, the set of probes could identify the recently described species, B. bavariensis.
在欧洲，已在蓖子硬蜱（Ixodes ricinus）中报告了属于伯氏疏螺旋体复合种的 7 种伯氏螺旋体。此外，还描述了另一种与回归热螺旋体相关的伯氏螺旋体。在本研究中，我们设计了反向线杂交探针，允许在扩增 23S 和 5S 核糖体基因之间的可变间隔区后检测和鉴定所有这些伯氏螺旋体。这些新的探针使我们能够调查在瑞士绍蒙（Chaumont）南坡采集的 915 只蓖子硬蜱中的伯氏螺旋体多样性。在 159 只感染的蜱中，鉴定出了 7 种伯氏螺旋体，并且首次在该地区鉴定出了 B. spielmanii 和回归热样螺旋体。B. valaisiana 和 B. spielmanii 在雄性蜱中明显比雌性或若虫蜱中存在得少。在 4 只蜱中检测到 RFL 螺旋体和莱姆病螺旋体的混合感染。此外，该探针集还可以鉴定最近描述的物种 B. bavariensis。
