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24-表油菜素内酯，一种来自油菜素甾体家族的植物甾醇，可保护多巴胺能细胞免受MPP诱导的氧化应激和细胞凋亡。

24-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP-Induced Oxidative Stress and Apoptosis.

作者信息

Carange Julie, Longpré Fanny, Daoust Benoit, Martinoli Maria-Grazia

机构信息

Department of Biochemistry, Neurosciences Research Group, Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada G9A 5H7.

出版信息

J Toxicol. 2011;2011:392859. doi: 10.1155/2011/392859. Epub 2011 Jun 3.

DOI:10.1155/2011/392859
PMID:21776258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3135132/
Abstract

Oxidative stress and apoptosis are frequently cited to explain neuronal cell damage in various neurodegenerative disorders, such as Parkinson' s disease. Brassinosteroids (BRs) are phytosterols recognized to promote stress tolerance of vegetables via modulation of the antioxidative enzyme cascade. However, their antioxidative effects on mammalian neuronal cells have never been examined so far. We analyzed the ability of 24-epibrassinolide (24-Epi), a natural BR, to protect neuronal PC12 cells from 1-methyl-4-phenylpyridinium- (MPP(+)-) induced oxidative stress and consequent apoptosis in dopaminergic neurons. Our results demonstrate that 24-Epi reduces the levels of intracellular reactive oxygen species and modulates superoxide dismutase, catalase, and glutathione peroxidase activities. Finally, we determined that the antioxidative properties of 24-Epi lead to the inhibition of MPP(+)-induced apoptosis by reducing DNA fragmentation as well as the Bax/Bcl-2 protein ratio and cleaved caspase-3. This is the first time that the potent antioxidant and neuroprotective role of 24-Epi has been shown in a mammalian neuronal cell line.

摘要

氧化应激和细胞凋亡常被引用来解释各种神经退行性疾病（如帕金森病）中的神经元细胞损伤。油菜素甾醇（BRs）是一种植物甾醇，已知可通过调节抗氧化酶级联反应来提高蔬菜的抗逆性。然而，迄今为止，它们对哺乳动物神经元细胞的抗氧化作用尚未得到研究。我们分析了天然BR 24-表油菜素内酯（24-Epi）保护神经元PC12细胞免受1-甲基-4-苯基吡啶鎓（MPP(+)）诱导的氧化应激以及由此导致的多巴胺能神经元凋亡的能力。我们的结果表明，24-Epi可降低细胞内活性氧水平，并调节超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。最后，我们确定24-Epi的抗氧化特性通过减少DNA片段化以及Bax/Bcl-2蛋白比率和裂解的半胱天冬酶-3来抑制MPP(+)诱导的细胞凋亡。这是首次在哺乳动物神经元细胞系中证明24-Epi具有强大的抗氧化和神经保护作用。

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