构象转变蛋白的天然状态转换需要整体展开。
Native-state interconversion of a metamorphic protein requires global unfolding.
机构信息
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.
出版信息
Biochemistry. 2011 Aug 23;50(33):7077-9. doi: 10.1021/bi200750k. Epub 2011 Jul 26.
Abstract
Lymphotactin (Ltn) is a unique chemokine that under physiological solution conditions displays large-scale structural heterogeneity, defining a new category of "metamorphic proteins". Previous Ltn studies have indicated that each form is required for proper function, but the mechanism of interconversion remains unknown. Here we have investigated the temperature dependence of kinetic rates associated with interconversion and unfolding by stopped-flow fluorescence to determine transition-state free energies. Comparisons of derived thermodynamic parameters revealed striking similarities between interconversion and protein unfolding. We conclude that Ltn native-state rearrangement proceeds by way of a large-scale unfolding process rather than a unique intermediate structure.
摘要
淋巴细胞趋化因子 (Ltn) 是一种独特的趋化因子，在生理溶液条件下显示出大规模的结构异质性，定义了一个新的“变形蛋白”类别。先前的 Ltn 研究表明，每种形式都是正常功能所必需的，但转换机制仍然未知。在这里，我们通过停流荧光法研究了与转换和展开相关的动力学速率对温度的依赖性，以确定过渡态自由能。衍生热力学参数的比较表明，转换和蛋白质展开之间存在惊人的相似性。我们的结论是，Ltn 天然状态的重排是通过大规模展开过程而不是独特的中间结构进行的。
相似文献
1
Native-state interconversion of a metamorphic protein requires global unfolding.构象转变蛋白的天然状态转换需要整体展开。
Biochemistry. 2011 Aug 23;50(33):7077-9. doi: 10.1021/bi200750k. Epub 2011 Jul 26.
2
Electrostatic optimization of the conformational energy landscape in a metamorphic protein.静电优化构象能景观在一个变形蛋白。
Biochemistry. 2012 Nov 13;51(45):9067-75. doi: 10.1021/bi300842j. Epub 2012 Nov 2.
3
An engineered second disulfide bond restricts lymphotactin/XCL1 to a chemokine-like conformation with XCR1 agonist activity.一个工程化的二硫键将淋巴细胞趋化因子/XCL1限制为具有XCR1激动剂活性的趋化因子样构象。
Biochemistry. 2007 Mar 13;46(10):2564-73. doi: 10.1021/bi602365d. Epub 2007 Feb 16.
4
Chapter 3. Lymphotactin structural dynamics.第3章。淋巴细胞趋化因子的结构动力学。
Methods Enzymol. 2009;461:51-70. doi: 10.1016/S0076-6879(09)05403-2.
5
Dynamic interchanging native states of lymphotactin examined by SNAPP-MS.通过 SNAPP-MS 考察淋巴细胞趋化因子天然状态的动态转换
J Am Soc Mass Spectrom. 2011 Mar;22(3):399-407. doi: 10.1007/s13361-010-0042-3. Epub 2011 Jan 15.
6
Evidence for a Shared Mechanism in the Formation of Urea-Induced Kinetic and Equilibrium Intermediates of Horse Apomyoglobin from Ultrarapid Mixing Experiments.超快速混合实验证明马脱辅基肌红蛋白尿素诱导的动力学和平衡中间体形成过程存在共同机制。
PLoS One. 2015 Aug 5;10(8):e0134238. doi: 10.1371/journal.pone.0134238. eCollection 2015.
7
Folding of horse cytochrome c in the reduced state.还原态马细胞色素c的折叠
J Mol Biol. 2001 Oct 5;312(5):1135-60. doi: 10.1006/jmbi.2001.4993.
8
Structural rearrangement of human lymphotactin, a C chemokine, under physiological solution conditions.人淋巴细胞趋化因子（一种C类趋化因子）在生理溶液条件下的结构重排。
J Biol Chem. 2002 May 17;277(20):17863-70. doi: 10.1074/jbc.M200402200. Epub 2002 Mar 11.
9
Kinetic mechanism of folding and unfolding of Rhodobacter capsulatus cytochrome c2.荚膜红细菌细胞色素c2折叠与去折叠的动力学机制
Biochemistry. 1996 Dec 24;35(51):16852-62. doi: 10.1021/bi961976k.
10
Kinetics and motional dynamics of spin-labeled yeast iso-1-cytochrome c: 1. Stopped-flow electron paramagnetic resonance as a probe for protein folding/unfolding of the C-terminal helix spin-labeled at cysteine 102.自旋标记酵母异-1-细胞色素c的动力学和运动动力学：1. 停流电子顺磁共振作为探测在半胱氨酸102处自旋标记的C端螺旋蛋白质折叠/去折叠的探针
Biochemistry. 1997 Mar 11;36(10):2884-97. doi: 10.1021/bi962155i.
引用本文的文献
1
Large-scale predictions of alternative protein conformations by AlphaFold2-based sequence association.基于AlphaFold2序列关联的替代蛋白质构象的大规模预测。
Nat Commun. 2025 Jul 1;16(1):5622. doi: 10.1038/s41467-025-60759-5.
2
The conformational landscape of fold-switcher KaiB is tuned to the circadian rhythm timescale.折叠开关 KaiB 的构象景观被调整到了生物钟的时间尺度。
Proc Natl Acad Sci U S A. 2024 Nov 5;121(45):e2412293121. doi: 10.1073/pnas.2412293121. Epub 2024 Oct 30.
3
Exploring the structural acrobatics of fold-switching proteins using simplified structure-based models.使用基于简化结构的模型探索折叠转换蛋白的结构杂技。
Biophys Rev. 2023 Jul 14;15(4):787-799. doi: 10.1007/s12551-023-01087-0. eCollection 2023 Aug.
4
Evolutionary selection of proteins with two folds.具有两种折叠结构的蛋白质的进化选择。
Nat Commun. 2023 Sep 6;14(1):5478. doi: 10.1038/s41467-023-41237-2.
5
Heterodimers Are an Integral Component of Chemokine Signaling Repertoire.异源二聚体是趋化因子信号谱的一个组成部分。
Int J Mol Sci. 2023 Jul 19;24(14):11639. doi: 10.3390/ijms241411639.
6
Fluid protein fold space and its implications.流体蛋白质折叠空间及其意义。
Bioessays. 2023 Sep;45(9):e2300057. doi: 10.1002/bies.202300057. Epub 2023 Jul 11.
7
Distinguishing features of fold-switching proteins.折叠转换蛋白的特征。
Protein Sci. 2023 Mar;32(3):e4596. doi: 10.1002/pro.4596.
8
Reversible switching between two common protein folds in a designed system using only temperature.仅通过温度在设计系统中实现两种常见蛋白质折叠构象的可逆转换。
Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2215418120. doi: 10.1073/pnas.2215418120. Epub 2023 Jan 20.
9
Structural and thermodynamic analyses of the β-to-α transformation in RfaH reveal principles of fold-switching proteins.RfaH 中β到α的转变的结构和热力学分析揭示了折叠转换蛋白的原理。
Elife. 2022 Oct 18;11:e76630. doi: 10.7554/eLife.76630.
10
Fragile protein folds: Sequence and environmental factors affecting the equilibrium of two interconverting, stably folded protein conformations.脆弱的蛋白质折叠：影响两种相互转化、稳定折叠的蛋白质构象平衡的序列和环境因素。
Magn Reson (Gott). 2021;2(1):63-76. doi: 10.5194/mr-2-63-2021. Epub 2021 Mar 10.
本文引用的文献
1
Lymphotactin: how a protein can adopt two folds.淋巴趋化因子：一种蛋白如何呈现两种构象。
J Chem Phys. 2009 Dec 28;131(24):245105. doi: 10.1063/1.3276284.
2
Slow transition between two beta-strand registers is dictated by protein unfolding.两个β-链结构域之间的缓慢转变由蛋白质解折叠决定。
J Am Chem Soc. 2009 Aug 19;131(32):11306-7. doi: 10.1021/ja9048338.
3
Chapter 3. Lymphotactin structural dynamics.第3章。淋巴细胞趋化因子的结构动力学。
Methods Enzymol. 2009;461:51-70. doi: 10.1016/S0076-6879(09)05403-2.
4
How chemokines invite leukocytes to dance.趋化因子如何吸引白细胞“翩翩起舞”。
Nat Immunol. 2008 Sep;9(9):953-9. doi: 10.1038/ni.f.207.
5
Biochemistry. Metamorphic proteins.生物化学。变质蛋白。
Science. 2008 Jun 27;320(5884):1725-6. doi: 10.1126/science.1158868.
6
Interconversion between two unrelated protein folds in the lymphotactin native state.淋巴趋化因子天然状态下两种不相关蛋白质折叠之间的相互转换。
Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5057-62. doi: 10.1073/pnas.0709518105. Epub 2008 Mar 25.
7
An engineered second disulfide bond restricts lymphotactin/XCL1 to a chemokine-like conformation with XCR1 agonist activity.一个工程化的二硫键将淋巴细胞趋化因子/XCL1限制为具有XCR1激动剂活性的趋化因子样构象。
Biochemistry. 2007 Mar 13;46(10):2564-73. doi: 10.1021/bi602365d. Epub 2007 Feb 16.
8
Identification and characterization of a glycosaminoglycan recognition element of the C chemokine lymphotactin.C趋化因子淋巴细胞趋化素的糖胺聚糖识别元件的鉴定与表征
J Biol Chem. 2004 Mar 26;279(13):12598-604. doi: 10.1074/jbc.M311633200. Epub 2004 Jan 5.
9
Glycosaminoglycan binding and oligomerization are essential for the in vivo activity of certain chemokines.糖胺聚糖结合和寡聚化对于某些趋化因子的体内活性至关重要。
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1885-90. doi: 10.1073/pnas.0334864100. Epub 2003 Feb 5.
10
Structural rearrangement of human lymphotactin, a C chemokine, under physiological solution conditions.人淋巴细胞趋化因子（一种C类趋化因子）在生理溶液条件下的结构重排。
J Biol Chem. 2002 May 17;277(20):17863-70. doi: 10.1074/jbc.M200402200. Epub 2002 Mar 11.
Zotero 插件