ESI-MS 分析结核分枝杆菌细胞壁抗原 85 酶，可进行底物谱分析和设计基于机制的抑制剂。
ESI-MS assay of M. tuberculosis cell wall antigen 85 enzymes permits substrate profiling and design of a mechanism-based inhibitor.
机构信息
Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK.
出版信息
J Am Chem Soc. 2011 Aug 31;133(34):13232-5. doi: 10.1021/ja204249p. Epub 2011 Aug 9.
Abstract
Mycobacterium tuberculosis Antigen 85 enzymes are vital to the integrity of the highly impermeable cell envelope and are potential therapeutic targets. Kinetic analysis using a label-free assay revealed both mechanistic details and a substrate profile that allowed the design and construction of a selective in vitro mechanism-based inhibitor.
摘要
结核分枝杆菌抗原 85 酶对于高度不可渗透的细胞包膜的完整性至关重要，是潜在的治疗靶点。使用无标记分析的动力学分析揭示了机制细节和底物特征，从而可以设计和构建选择性的体外基于机制的抑制剂。
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