对豚鼠巨细胞病毒多肽的免疫反应及与人巨细胞病毒的交叉反应性。

Immune response to guinea pig cytomegalovirus polypeptides and cross reactivity with human cytomegalovirus.

作者信息

Kacica M A, Harrison C J, Myers M G, Bernstein D I

机构信息

Division of Infectious Diseases, Children's Hospital Research Foundation, Cincinnati, Ohio 45229-2899.

出版信息

J Med Virol. 1990 Nov;32(3):155-9. doi: 10.1002/jmv.1890320305.

DOI:10.1002/jmv.1890320305

PMID:2177780

Abstract

The immune response to guinea pig cytomegalovirus (gpCMV) was evaluated by immunoblotting. Preinoculation guinea pig plasma did not react with gpCMV antigen, whereas convalescent plasma reacted to at least 18 gpCMV-specific polypeptides. The initial immune response was primarily directed at polypeptides with MWs of 100, 75, and 56 kDa. Over 80% of plasma collected more than 29 days after viral inoculation reacted to these polypeptides and also to those with MW of 54, 52, and 38 kDa. In this report, we also demonstrate cross reactivity between gpCMV and human CMV (HCMV). Human immunoglobulin (IVIG) reacted to at least 20 HCMV polypeptides and cross reacted with six gpCMV polypeptides. GpCMV convalescent plasma also reacted with HCMV polypeptides.

摘要

通过免疫印迹法评估了豚鼠对豚鼠巨细胞病毒（gpCMV）的免疫反应。接种前的豚鼠血浆不与gpCMV抗原发生反应，而恢复期血浆与至少18种gpCMV特异性多肽发生反应。最初的免疫反应主要针对分子量为100、75和56 kDa的多肽。病毒接种后29天以上采集的血浆中，超过80%与这些多肽以及分子量为54、52和38 kDa的多肽发生反应。在本报告中，我们还证明了gpCMV与人巨细胞病毒（HCMV）之间的交叉反应性。人免疫球蛋白（IVIG）与至少20种HCMV多肽发生反应，并与6种gpCMV多肽发生交叉反应。gpCMV恢复期血浆也与HCMV多肽发生反应。