由解淀粉芽孢杆菌 GRAS 微生物从葡萄糖生产 2,3-丁二醇。
Production of 2,3-butanediol from glucose by GRAS microorganism Bacillus amyloliquefaciens.
机构信息
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province 214122, PR China.
出版信息
J Basic Microbiol. 2011 Dec;51(6):650-8. doi: 10.1002/jobm.201100033. Epub 2011 Jul 21.
In the current study, a GRAS (Generally Recognized As Safe) strain of Bacillus amyloliquefaciens producing 2,3-butanediol (2,3-BD) designated as B10-127 was isolated in our lab. The strain B10-127 produced 2,3-BD effectively under the condition of 20% glucose (quality concentration), showed a high-glucose tolerance. The effects of initial glucose concentration, temperature, pH and agitation on 2,3-BD production were investigated in this work and the proper parameters were identified. Accordingly, the fed-batch culture of B10-127 in larger scales (5 l) showed a remarkable 2,3-BD producing potency. The maximum 2,3-BD concentration reached 92.3 g/l at 96 h with a 2,3-BD productivity of 0.96 g/l h. To our knowledge, the results were new records on 2,3-BD fermentation by Bacillus, which shown an excellent candidate for the microbial fermentation of 2,3-BD on an industrial scale.
在本研究中，我们实验室分离到一株能够产生 2,3-丁二醇（2,3-BD）的 GRAS（一般认为安全）解淀粉芽孢杆菌菌株，命名为 B10-127。在 20%葡萄糖（质量浓度）的条件下，菌株 B10-127 能够有效地生产 2,3-BD，并且具有较高的耐葡萄糖能力。本研究考察了初始葡萄糖浓度、温度、pH 值和搅拌速度对 2,3-BD 生产的影响，并确定了合适的参数。因此，在较大规模（5L）下对 B10-127 进行分批补料培养显示出了显著的 2,3-BD 生产能力。在 96 h 时，2,3-BD 的最大浓度达到 92.3 g/L，2,3-BD 的生产速率为 0.96 g/L/h。据我们所知，这些结果是芽孢杆菌发酵 2,3-BD 的新纪录，表明其是工业规模微生物发酵 2,3-BD 的优秀候选菌株。
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