Department of Physiology, University of Medicine and Pharmacy Victor Babes Timisoara, Timisoara, Romania.
J Cell Mol Med. 2011 Oct;15(10):2269-72. doi: 10.1111/j.1582-4934.2011.01386.x.
Human skeletal muscle tissue displays specific cellular architecture easily damaged during individual existence, requiring multiple resources for regeneration. Congruent with local prerequisites, heterogeneous muscle stem cells (MuSCs) are present in the muscle interstitium. In this study, we aimed to characterize the properties of human muscle interstitial cells that had the characteristic morphology of telocytes (TCs). Immunocytochemistry and immunofluorescence showed that cells with TC morphology stained positive for c-kit/CD117 and VEGF. C-kit positive TCs were separated with magnetic-activated cell sorting, cultured in vitro and expanded for study. These cells exhibited high proliferation capacity (60% expressed endoglin/CD105 and 80% expressed nuclear Ki67). They also exhibited pluripotent capacity limited to Oct4 nuclear staining. In addition, 90% of c-kit positive TCs expressed VEGF. C-kit negative cells in the MuSCs population exhibited fibroblast-like morphology, low trilineage differential potential and negative VEGF staining. These results suggested that c-kit/CD117 positive TCs represented a unique cell type within the MuSC niche.
人类骨骼肌组织具有独特的细胞结构，在个体存在期间容易受损，需要多种资源来进行再生。与局部先决条件一致，肌肉间质中存在异质性的肌肉干细胞（MuSCs）。在这项研究中，我们旨在表征具有间质细胞特征形态的人类肌肉间质细胞的特性，这些细胞具有长梭形的 telocytes（TCs）形态。免疫细胞化学和免疫荧光显示，具有 TC 形态的细胞染色阳性，表达 c-kit/CD117 和 VEGF。使用磁激活细胞分选分离 c-kit 阳性 TCs，进行体外培养和扩增以进行研究。这些细胞表现出高增殖能力（60%表达 endoglin/CD105，80%表达核 Ki67）。它们还表现出有限的多能性，仅限于核 Oct4 染色。此外，90%的 c-kit 阳性 TCs 表达 VEGF。MuSCs 群体中的 c-kit 阴性细胞表现出成纤维细胞样形态、低三系分化潜能和阴性 VEGF 染色。这些结果表明，c-kit/CD117 阳性 TCs 代表 MuSC 生态位中的一种独特细胞类型。
