Department of Biology, Unit of Pollution and Ecotoxicology, Section of Zoology, University of Patras, Rio 26500, Greece.
Environ Toxicol Pharmacol. 2004 May;17(1):9-12. doi: 10.1016/j.etap.2004.01.003.
Ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. This paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, Sparus aurata and accumulate in the brain. The concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive ELISA commercial kit. Our results showed a rapid uptake of the substance by the brain of the fish reaching a maximum concentration of 98.9ngg(-1) 8h post treatment. The trend of the absorption of the drug in brain followed that of the blood. Concentration of the drug in the brain remained high at each sampling point over the 24h duration of the experiment. In view of these findings, the need of study of the role of the blood-brain barrier and particularly the multidrug resistance mechanism in sea bream is outlined.
伊维菌素在兽医和人类中被广泛使用，由于其无法穿透高等脊椎动物的中枢神经系统，因此被认为是安全的。本文介绍了该药物能够穿过海水养殖真鲷（Sparus aurata）的血脑屏障并在大脑中积累的能力的数据。使用直接竞争 ELISA 商业试剂盒评估了药物在鱼脑和血清中的浓度。我们的结果表明，该物质被鱼的大脑快速吸收，在治疗后 8 小时达到 98.9ngg(-1)的最大浓度。药物在大脑中的吸收趋势与血液中的吸收趋势一致。在实验的 24 小时内，药物在大脑中的浓度在每个采样点都保持较高水平。鉴于这些发现，概述了研究血脑屏障特别是多药耐药机制在真鲷中的作用的必要性。
