Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, Tamil Nadu, India.
Biochem Biophys Res Commun. 2011 Aug 12;411(4):661-6. doi: 10.1016/j.bbrc.2011.06.167. Epub 2011 Jul 13.
The present study has evaluated the chemopreventive effects of gossypol on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis and on human gastric adenocarcinoma (AGS) cell line. Gossypol, C(30)H(30)O(8), is a polyphenolic compound that has anti proliferative effect and induces apoptosis in various cancer cells. The aim of this work was to delineate in vivo and in vitro anti-initiating mechanisms of orally administered gossypol in target (stomach) tissues and in human gastric adenocarcinoma (AGS) cell line. In vitro results prove that gossypol has potent cytotoxic effect and inhibit the proliferation of adenocarcinoma (AGS) cell line. In vivo results prove gossypol to be successful in prolonging the survival of MNNG induced cancer bearing animals and in delaying the onset of tumor in animals administrated with gossypol and MNNG simultaneously. Examination of the target (stomach) tissues in sacrificed experimental animals shows that administration of gossypol significantly reduces the level of tumor marker enzyme (carcino embryonic antigen) and pepsin. The level of Nucleic acid contents (DNA and RNA) significantly reduces, and the membrane damage of glycoprotein subsides, in the target tissues of cancer bearing animals, with the administration of gossypol. These data suggest that gossypol may create a beneficial effect in patients with gastric cancer.
本研究评价了棉酚对 N-甲基-N'-硝基-N-亚硝基胍（MNNG）诱导的胃癌发生的化学预防作用，以及对人胃腺癌（AGS）细胞系的作用。棉酚，C(30)H(30)O(8)，是一种多酚化合物，具有抗增殖作用，并诱导各种癌细胞凋亡。本工作的目的是阐明口服棉酚在靶（胃）组织和人胃腺癌（AGS）细胞系中的体内和体外起始抑制机制。体外结果证明棉酚具有很强的细胞毒性作用，并抑制腺癌（AGS）细胞系的增殖。体内结果证明棉酚能成功延长 MNNG 诱导的癌症荷瘤动物的存活时间，并延迟同时给予棉酚和 MNNG 的动物肿瘤的发生。对处死的实验动物靶（胃）组织的检查表明，棉酚的给药显著降低了肿瘤标志物酶（癌胚抗原）和胃蛋白酶的水平。荷瘤动物靶组织中核酸含量（DNA 和 RNA）显著降低，糖蛋白的膜损伤减轻。这些数据表明，棉酚可能对胃癌患者产生有益的影响。
