Epidemiology Branch, Division of Epidemiology, Statistics, and Prevention Research, National Institute of Child Health and Human Development, 6100 Executive Blvd., Rockville, MD 20852, USA.
Environ Toxicol Pharmacol. 2005 Feb;19(2):203-13. doi: 10.1016/j.etap.2004.04.009.
Much of the available literature focusing on organochlorine exposure and human health effects has relied upon serum for quantifying exposure despite adipose tissue being the purported "gold standard". The accuracy of exposure status is dependent upon serum being a valid and reliable proxy for adipose tissue regardless of compound under study and served as the impetus for study. Serum and omentum fat concentrations for 62 polychlorinated biphenyls (PCBs) and 7 organochlorine pesticides (OCPs) were determined using gas chromatography with electron capture and compared to assess their relative abundance and correlation among 15 women aged 18-40 years undergoing laparoscopy. The relation between concentration in serum and fat was determined by linear regression. Of the 20 organochlorines (OCs) (29%) present in both serum and fat samples, moderate linear correlations (r > 0.6) were observed between lipid-adjusted serum and fat concentrations for PCBs #138, 153, 180, 188, 194, 206, and DDE. Forty-nine OCs were present in adipose samples but measured below the LOD in serum samples. Our findings underscore the potential for discrepant human health results associated with OC exposure on the basis of medium used for quantification purposes, especially for less ubiquitous compounds or when study samples include individuals with relatively low exposures. These data support earlier findings and argue for concerted methodological work aimed at developing standardized laboratory methods for epidemiologic studies.
尽管脂肪组织被认为是“黄金标准”，但大多数关注有机氯暴露与人类健康影响的现有文献都依赖血清来量化暴露情况。暴露状况的准确性取决于血清是否可以作为脂肪组织的有效且可靠的替代物，而与研究中的化合物无关，这也是本研究的动力。采用带有电子捕获的气相色谱法测定了 62 种多氯联苯（PCBs）和 7 种有机氯农药（OCPs）在 15 名 18-40 岁接受腹腔镜检查的女性血清和网膜脂肪中的浓度，以评估它们的相对丰度和相关性。通过线性回归确定血清和脂肪中浓度之间的关系。在所研究的 20 种有机氯化合物（OCs）（29%）中，有 20 种存在于血清和脂肪样本中，在调整脂质后，血清和脂肪中 PCB#138、153、180、188、194、206 和 DDE 的浓度之间观察到中等线性相关性（r > 0.6）。49 种 OCs 存在于脂肪样本中，但在血清样本中低于检测限。我们的研究结果强调了基于定量目的所使用的介质，OC 暴露与人类健康结果之间可能存在差异，尤其是对于不太普遍的化合物，或者当研究样本包括暴露水平相对较低的个体时。这些数据支持了早期的研究结果，并呼吁开展协同的方法学工作，旨在为流行病学研究制定标准化的实验室方法。
