Yu Jian Qing, Liao Zhi Xiong, Cai Xiao Qiang, Lei Jia Chuan, Zou Guo Lin
College of Pharmacy, Wuhan University, Wuhan 430072, People's Republic of China; Renmin Hospital, Wuhan University, Wuhan 430060, People's Republic of China.
Environ Toxicol Pharmacol. 2007 Mar;23(2):162-7. doi: 10.1016/j.etap.2006.08.004. Epub 2006 Aug 18.
The compositions of the essential oils from the rhizome and the aerial part of Aristolochia mollissima were analysed by GC-MS, 68 constituents (88.2% of the total oil) and 74 constituents (89.4% of the total oil) were identified, respectively. 2,2,7,7-Tetramethyltricyclo[6.2.1.0(1,6)]undec-4-en-3-one was the most abundant constituent among all in the ratios of 15.9% and 13.5% from the rhizome and the aerial part of A. mollissima, respectively. Among other main compounds, (E)-β-santalol acetate (10.3%) and camphene (6.7%) were detected in the rhizome oil, spathulenol (6.8%) was detected in the oil from the aerial par of A. mollissima. The antimicrobial activity of the essential oils from the rhizome and the aerial part of A. mollissima was evaluated against 20 microorganisms using disc diffusion and broth microdilution methods. The gram-positive bacteria were more sensitive to both oils than gram-negative bacteria and yeasts. The rhizome oil showed the strongest bactericidal activity against Staphylococcus saprophyticus, whereas the oil from the aerial part of A. mollissima exerted the strongest bactericidal activity against methicillin-resistant and methicillin-senstive Staphylococcus aureus. The in vitro cytotoxicity of both oils on six human cancer cell lines were also examined. The cytotoxicity of the rhizome oil on four cancer cell lines (ACHN, Bel-7402, Hep G2 and HeLa) was significantly stronger than that of the oil from the aerial part of A. mollissima.
采用气相色谱 - 质谱联用（GC - MS）技术分析了绵毛马兜铃根茎和地上部分挥发油的成分，分别鉴定出68种成分（占挥发油总量的88.2%）和74种成分（占挥发油总量的89.4%）。2,2,7,7 - 四甲基三环[6.2.1.0(1,6)]十一碳 - 4 - 烯 - 3 - 酮是所有成分中含量最高的，在绵毛马兜铃根茎和地上部分中的比例分别为15.9%和13.5%。在其他主要化合物中，根茎挥发油中检测到（E）-β - 檀香醇乙酸酯（10.3%）和莰烯（6.7%），绵毛马兜铃地上部分挥发油中检测到匙叶桉油烯醇（6.8%）。采用纸片扩散法和肉汤微量稀释法，评价了绵毛马兜铃根茎和地上部分挥发油对20种微生物的抗菌活性。革兰氏阳性菌对两种挥发油的敏感性高于革兰氏阴性菌和酵母菌。根茎挥发油对腐生葡萄球菌表现出最强的杀菌活性，而绵毛马兜铃地上部分挥发油对耐甲氧西林和甲氧西林敏感的金黄色葡萄球菌表现出最强的杀菌活性。还检测了两种挥发油对六种人癌细胞系的体外细胞毒性。根茎挥发油对四种癌细胞系（ACHN、Bel - 7402、Hep G2和HeLa）的细胞毒性明显强于绵毛马兜铃地上部分挥发油。
