多花山竹子（Roxb.）Bedd. 醇提物的抗氧化、细胞毒性和遗传毒性评价

Antioxidant, cytotoxic and genotoxic evaluation of alcoholic extract of Polyalthia cerasoides (Roxb.) Bedd.

机构信息

Department of P.G. Studies and Research in Chemistry, School of Chemical Sciences, Kuvempu University, Shankaraghatta, 577451 Karnataka, India.

出版信息

Environ Toxicol Pharmacol. 2008 Sep;26(2):142-6. doi: 10.1016/j.etap.2008.03.001. Epub 2008 Mar 16.

DOI:10.1016/j.etap.2008.03.001

PMID:21783902

Abstract

Antioxidative potential of alcohol extract of Polyalthia cerasoides was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical, superoxide anion scavenging, and reducing power assays were performed. The extract showed significant reactive oxygen species (ROS) scavenging activity in all in vitro antioxidant assays and contained high level of total phenolic content. For in vivo genotoxic evaluation, Swiss albino mice were treated with alcohol extract at the concentration of 40mg/kg body weight. Frequency of aberration was compared with control. Both the sets did not showed genotoxic effect. Further the extract was subjected to cytotoxic study using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay, the extract confirmed to show moderate cytotoxicity against L929 cell line.

摘要

采用 1,1-二苯基-2-苦基肼基（DPPH）、羟基自由基、超氧阴离子清除和还原力测定法评价鹿角藤醇提物的抗氧化潜力。该提取物在所有体外抗氧化测定中均表现出显著的活性氧（ROS）清除活性，且含有高水平的总酚含量。为了进行体内遗传毒性评价，将瑞士白化病小鼠用 40mg/kg 体重的醇提取物处理。将畸变频率与对照组进行比较。两组均未显示出遗传毒性作用。进一步用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法对提取物进行细胞毒性研究，提取物对 L929 细胞系表现出中等的细胞毒性。