Laboratory of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
Mol Biol Rep. 2012 Apr;39(4):3971-6. doi: 10.1007/s11033-011-1177-4. Epub 2011 Jul 24.
CD163 and sialoadhesin (SN) were reported as two essential receptors for the porcine reproductive and respiratory syndrome virus. To investigate the relationship between these two genes and porcine immunity, we assigned porcine CD163 and SN respectively to SSC5q21-q24 and SSC17q23 by IMpRH. Expression profiles revealed that CD163 and SN were ubiquitously expressed in ten tissues, and were expressed highly in lymph gland, spleen and liver, which implied the potential functions of CD163 and SN in immunity. Moreover, a single nucleotide polymorphism (SNP) c.3534C>T was found in 3'-UTR of the CD163 gene and association analysis showed that this gene was significantly associated with the IgG content in blood (P < 0.05). A novel missense mutation c.878A>G located in exon4 of the SN gene which caused the amino acid transition from histidine to arginine was detected, and it was significantly associated with the WBC count in the peripheral blood (P < 0.05). These results provided fundamental evidence for CD163 and SN as two functional candidate genes affecting immunity in pigs.
CD163 和唾液酸黏附素 (SN) 被报道为猪繁殖与呼吸综合征病毒的两个重要受体。为了研究这两个基因与猪免疫之间的关系，我们通过 IMpRH 将猪 CD163 和 SN 分别定位到 SSC5q21-q24 和 SSC17q23。表达谱显示，CD163 和 SN 在十种组织中广泛表达，在淋巴结、脾脏和肝脏中表达水平较高，这表明 CD163 和 SN 在免疫中可能具有潜在的功能。此外，在 CD163 基因的 3'-UTR 中发现了一个单核苷酸多态性 (SNP) c.3534C>T，关联分析表明该基因与血液中的 IgG 含量显著相关 (P < 0.05)。在 SN 基因的外显子 4 中检测到一个新的错义突变 c.878A>G，导致组氨酸到精氨酸的氨基酸转换，该突变与外周血白细胞计数显著相关 (P < 0.05)。这些结果为 CD163 和 SN 作为影响猪免疫的两个功能候选基因提供了基础证据。
