巨大曲霉对潮霉素B的抗性转化
Transformation of Aspergillus giganteus to hygromycin B resistance.
作者信息
Wnendt S, Jacobs M, Stahl U
机构信息
Technische Universität Berlin, Fachgebiet für Mikrobiologie and Genetik.
出版信息
Curr Genet. 1990 Jan;17(1):21-4. doi: 10.1007/BF00313244.
A wild strain of A. giganteus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of A. nidulans sequences. Stable transformants arose by heterogenous integration, mainly of tandem repeats of vector DNA at various sites in the host genome. Between 6 and 30 resistant colonies were obtained per microgram DNA per 3 x 10(3) viable protoplasts. Vector DNA could be recovered by transformation of Escherichia coli with undigested genomic DNA from Aspergillus giganteus transformants.
利用构巢曲霉序列控制下的细菌抗性基因，将巨大曲霉的野生菌株转化为对潮霉素B具有抗性。稳定的转化体通过异源整合产生，主要是载体DNA在宿主基因组不同位点的串联重复。每3×10³个活原生质体每微克DNA可获得6至30个抗性菌落。通过用来自巨大曲霉转化体的未消化基因组DNA转化大肠杆菌，可以回收载体DNA。
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