Ruttkay-Nedecký B, Subík J
Food Research Institute, Bratislava, Czechoslovakia.
Curr Genet. 1990 Jan;17(1):85-8. doi: 10.1007/BF00313254.
Ogd1 mutants of Saccharomyces cerevisiae are deficient in mitochondrial 2-oxoglutarate dehydrogenase activity; they cannot grow on glycerol and produce an increased amount of organic acids during growth on glucose as substrate. Using gamma ray-induced rad52-mediated chromosome loss the ogd1 mutation can be assigned to chromosome IX. Tetrad analysis of crosses between ogd1 and other markers on chromosome IX revealed that the OGD1 gene maps on the left arm of this chromosome 1.9 cM from his5.
酿酒酵母的Ogd1突变体线粒体2-氧代戊二酸脱氢酶活性存在缺陷；它们不能在甘油上生长，并且在以葡萄糖为底物生长期间会产生更多的有机酸。利用γ射线诱导的rad52介导的染色体丢失，可将ogd1突变定位到IX号染色体上。对ogd1与IX号染色体上其他标记之间杂交的四分体分析表明，OGD1基因位于该染色体左臂上，距his5为1.9 cM。
