Laboratory of Applied Physiology, Veterinary Sciences Department, Center for Agrarian Sciences, Federal University of Paraíba, Areia, PB, Brazil.
Clinics (Sao Paulo). 2011;66(5):873-8. doi: 10.1590/s1807-59322011000500026.
Chronic ethanol consumption is a major public health problem throughout the world. We investigated the anxiolytic-like effects and the possible ever injury induced by the chronic consumption of ethanol or sugarcane spirit in mice.
Adult mice were exposed to a two-bottle free-choice paradigm for 6 weeks. The mice in Group A (n = 16) had access to sugarcane spirit + distilled water, the mice in Group B (n = 15) had access to ethanol + distilled water, and the mice in Group C (control, n = 14) had access to distilled water + distilled water. The ethanol content in the beverages offered to Groups A and B was 2% for the first week, 5% for the second week and 10% for the remaining four weeks. At the end of the experimental period, the mice were evaluated using the elevated-plus maze and the hole-board test to assess their anxiety-related behaviors. We also determined the serum aspartate aminotransferase and alanine aminotransferase levels.
In the elevated-plus maze, the time spent in the open arms was increased in the mice exposed to chronic ethanol (32 ± 8 vs. 7 ± 2 s, n = 9) or sugarcane spirit (36 ± 9 vs. 7 ± 2 s, n = 9) compared to the controls. In the hole-board test, the mice exposed to ethanol or sugarcane spirit displayed increases in their head-dipping frequency (16 ± 1 for the control group, 27 ± 2 for the ethanol group, and 31 ± 3 for the sugarcane-spirit group; n = 9 for each group). In addition, the mice exposed to sugarcane spirit displayed an increase in the aspartate aminotransferase / alanine aminotransferase ratio compared to the ethanol group (1.29 ± 0.17 for the control group and 2.67 ± 0.17 for the sugarcane spirit group; n = 8 for each group).
The chronic consumption of sugarcane-spirit produces liver injury and anxiolytic-like effects and the possible liver injury in mice.
慢性乙醇摄入是全世界的一个主要公共卫生问题。我们研究了慢性乙醇或甘蔗酒摄入对小鼠的抗焦虑样作用和可能的肝损伤。
成年小鼠进行了为期 6 周的双瓶自由选择范式。A 组（n = 16）的小鼠可获得甘蔗酒+蒸馏水，B 组（n = 15）的小鼠可获得乙醇+蒸馏水，C 组（对照组，n = 14）的小鼠可获得蒸馏水+蒸馏水。A 组和 B 组提供的饮料中的乙醇含量分别在第一周为 2%，第二周为 5%，其余四周为 10%。在实验期末，通过高架十字迷宫和洞板试验评估小鼠的焦虑相关行为。我们还测定了血清天冬氨酸转氨酶和丙氨酸转氨酶水平。
在高架十字迷宫中，与对照组相比，暴露于慢性乙醇（32 ± 8 比 7 ± 2 s，n = 9）或甘蔗酒（36 ± 9 比 7 ± 2 s，n = 9）的小鼠在敞臂上的停留时间增加。在洞板试验中，暴露于乙醇或甘蔗酒的小鼠的头探频率增加（对照组 16 ± 1，乙醇组 27 ± 2，甘蔗酒组 31 ± 3；每组 n = 9）。此外，与乙醇组相比，暴露于甘蔗酒的小鼠的天冬氨酸转氨酶/丙氨酸转氨酶比值增加（对照组 1.29 ± 0.17，甘蔗酒组 2.67 ± 0.17；每组 n = 8）。
慢性甘蔗酒摄入可导致小鼠肝损伤和抗焦虑样作用，以及可能的肝损伤。
