Liu Jie, Jiang Yujia, Chen Tianpeng, Dong Weiliang, Zhang Wenming, Ma Jiangfeng, Jiang Min, Xin Fengxue
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816 People's Republic of China.
2Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211816 People's Republic of China.
3 Biotech. 2018 Oct;8(10):406. doi: 10.1007/s13205-018-1430-9. Epub 2018 Sep 14.
The genome of a wild-type solventogenic sp. strain LJ4 that could directly convert undetoxified lignocellulosic hydrolysate to butanol and tolerate high concentration of furan and phenolic derivates occurring in the lignocellulosic hydrolysate is described. 16S rDNA gene sequencing and analysis indicated that it is closely related to . The genome size of strain LJ4 is 3.90 Mp, which has a G + C content of 30.72% and encodes 2711 proteins. It also has one 0.19 Mp plasmid with 181 predicted encoding proteins. Alcohol dehydrogenases (ADs) and a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent flavin mononucleotide (FMN) reductase were identified, which may play key roles in inhibitors' resistance in lignocellulosic hydrolysate.
本文描述了野生型产溶剂梭菌属菌株LJ4的基因组，该菌株能够直接将未解毒的木质纤维素水解产物转化为丁醇，并耐受木质纤维素水解产物中高浓度的呋喃和酚类衍生物。16S rDNA基因测序与分析表明，它与……密切相关。菌株LJ4的基因组大小为3.90 Mp，G + C含量为30.72%，编码2711种蛋白质。它还拥有一个0.19 Mp的质粒，带有181个预测的编码蛋白。已鉴定出酒精脱氢酶（ADs）和一种烟酰胺腺嘌呤二核苷酸磷酸（NADPH）依赖性黄素单核苷酸（FMN）还原酶，它们可能在木质纤维素水解产物的抑制剂抗性中起关键作用。
