College of Animal Sciences, Zhejiang University, Kaixuan Road 268#, Hangzhou 310029, China.
Environ Toxicol Pharmacol. 2008 Nov;26(3):325-30. doi: 10.1016/j.etap.2008.06.008. Epub 2008 Jul 10.
The aim of the study was to investigate the protective effects of oxyresveratrol and cis-mulberroside A isolated from Ramulus mori on the liver of mice intoxicated with ethanol. Animals were pretreated with different doses (30 and 60mg/kg of body weight) of oxyresveratrol and cis-mulberroside A prior to the ethanol (9g/kg of body weight) orally for 7 days. Ethanol treatment induced the decrease of reduced glutathione level and antioxidant enzymes activities, the elevation of the lipid peroxidation and cytochrome P450 2E1 activity accompanied with the increase of iron concentration and mitochondrial permeability transition. Pretreatment with oxyresveratrol and cis-mulberroside A restored the changes in the above parameters up to the basal level. The protective effects of the two active compounds were further supported by attenuation of the degree of tissue damage and the regulation of the expression of TNF-α. It could be concluded that oxyresveratrol and cis-mulberroside A from R. mori could protect mice against ethanol-induced hepatic damage.
本研究旨在探讨从桑枝中分离得到的白藜芦醇苷和顺式 mulberroside A 对乙醇中毒小鼠肝脏的保护作用。动物在乙醇（9g/kg 体重）口服 7 天前，用不同剂量（30 和 60mg/kg 体重）的白藜芦醇苷和顺式 mulberroside A 预处理。乙醇处理导致还原型谷胱甘肽水平和抗氧化酶活性降低，脂质过氧化和细胞色素 P450 2E1 活性升高，铁浓度和线粒体通透性转换增加。用白藜芦醇苷和顺式 mulberroside A 预处理可将上述参数的变化恢复到基础水平。两种活性化合物的保护作用还得到了组织损伤程度减弱和 TNF-α 表达调节的支持。可以得出结论，桑枝中的白藜芦醇苷和顺式 mulberroside A 可防止乙醇引起的肝损伤。
