Devine D A, Roberts A P, Rowe B
Department of Medical Microbiology, Charing Cross and Westminster Medical School, London, England.
J Clin Pathol. 1990 Jan;43(1):76-8. doi: 10.1136/jcp.43.1.76.
The K1 antigen is a poor immunogen, its detection by serological means is difficult, and previously described methods using K1 specific bacteriophages require standardised suspensions of five different bacteriophages. A simple technique was developed which uses an unstandardised suspension of a single K1 specific bacteriophage applied with a 1 mm wire loop to bacteria streaked on to cystine lactose electrolyte deficient (CLED) medium. The technique correctly identified all 99 known K1 strains tested, including 14 strains negative with the serological method. Among 71 clinical isolates from urinary tract infections, the single bacteriophage method distinguished 30 K1 strains from 41 strains without this antigen. Suspensions of the bacteriophage were shown to remain fully active for at least two years when stored at 4 degrees C. It is concluded that this technique required so little in materials, time, or equipment that it could be routinely used in most laboratories.
K1抗原是一种弱免疫原，通过血清学方法检测它很困难，而且之前描述的使用K1特异性噬菌体的方法需要五种不同噬菌体的标准化悬液。开发了一种简单的技术，该技术使用单一K1特异性噬菌体的非标准化悬液，用1毫米的金属丝环将其应用于接种在胱氨酸乳糖电解质缺乏（CLED）培养基上的细菌。该技术正确鉴定了所有99株测试的已知K1菌株，包括14株血清学方法检测为阴性的菌株。在71株尿路感染临床分离株中，单一噬菌体方法区分出了30株带有K1抗原的菌株和41株没有该抗原的菌株。当保存在4℃时，噬菌体悬液至少两年仍保持完全活性。得出的结论是，该技术在材料、时间或设备方面所需极少，因此可以在大多数实验室常规使用。
