Prochownik E V, Panem S, Kirsten W H
J Gen Virol. 1979 Feb;42(2):399-403. doi: 10.1099/0022-1317-42-2-399.
The induction of a type C virus from a strain of human embryonic lung cells (HEL-12) by iododeoxyuridine (IdUrd) was examined at various times during in vitro propagation. IdUrd did not elicit type C virus production immediately following initiation of cultures from frozen primary HEL-12 cells. After overnight treatment with 30 microgram/ml IdUrd the cells expressed viral antigens and produced a type C virus between the 25th and 80th day of in vitro growth. Production of the induced type C virus was transient. Single-cell clones of the parental HEL-12 strain were likewise susceptible to type C virus production by IdUrd. The ability of IdUrd to induce virus terminated with the onset of spontaneous type C virus production from HEL-12 cells at between 80 and 120 days of in vitro growth.
在体外培养的不同时间，检测了碘脱氧尿苷（IdUrd）对一株人胚肺细胞（HEL-12）中C型病毒的诱导作用。从冷冻的原代HEL-12细胞开始培养后，IdUrd并不会立即引发C型病毒的产生。用30微克/毫升IdUrd过夜处理后，细胞在体外生长的第25天至第80天之间表达病毒抗原并产生了一种C型病毒。诱导产生的C型病毒的产生是短暂的。亲代HEL-12株的单细胞克隆同样易受IdUrd诱导产生C型病毒。在体外生长80至120天之间，当HEL-12细胞开始自发产生C型病毒时，IdUrd诱导病毒的能力就终止了。
