Biomicrofluidics. 2011 Jun;5(2):24117. doi: 10.1063/1.3605512. Epub 2011 Jun 30.
In this article, the authors present the fabrication of an enzyme-entrapped alginate hollow fiber using a microfluidic device. Further use of enzyme-entrapped alginate hollow fibers as a biocatalytic microchemical reactor for chemical synthesis is also deliberated in this article. To ensure that there is no enzyme leaching from the fiber, fiber surfaces were coated with chitosan. To confine the mobility of reactants and products within the porous hollow fibers the entire fibers were embedded into a transparent polydimethylsiloxane (PDMS) matrix which also works as a support matrix. A vanadium-containing bromoperoxidase enzyme isolated from Corallina confusa was used as a model enzyme to demonstrate the use of these alginate hollow-fiber reactors in bromo-oxidation of phenol red to bromophenol blue at different dye flow rates. Stability of the entrapped enzyme at different temperatures and the effect of the chitosan coating on the reaction conversion were also studied. It was observed that molecules as big as 27 kDa can be retained in the matrix after coating with chitosan while molecules with molecular-weight of around 378 Da can still diffuse in and out of the matrix. The kinetic conversion rate in this microfluidic bioreactor was more than 41-fold faster when compared with the standard test-tube procedure.
本文介绍了使用微流控装置制备包埋酶的海藻酸盐中空纤维。本文还讨论了将包埋酶的海藻酸盐中空纤维用作化学合成的生物催化微化学反应器。为了确保酶不从纤维中浸出，纤维表面用壳聚糖进行了涂层处理。为了限制反应物和产物在多孔中空纤维内的迁移，整个纤维被嵌入透明的聚二甲基硅氧烷（PDMS）基质中，该基质也可用作支撑基质。从珊瑚藻中分离出的含钒溴过氧化物酶被用作模型酶，以证明这些海藻酸盐中空纤维反应器在不同染料流速下用于苯酚红的溴氧化生成溴酚蓝。还研究了在不同温度下固定化酶的稳定性以及壳聚糖涂层对反应转化率的影响。观察到，用壳聚糖涂层后，分子量高达 27 kDa 的分子仍可保留在基质中，而分子量约为 378 Da 的分子仍可进出基质。与标准试管法相比，这种微流控生物反应器的动力学转化率快了 41 倍以上。
