Department of Pediatrics and Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. Burns School of Medicine, University of Hawai'i at Manoa, Honolulu, Hawaii; USA.
Am J Trop Med Hyg. 2011 Sep;85(3):540-5. doi: 10.4269/ajtmh.2011.11-0034.
Despite the recent discovery of genetically divergent hantaviruses in shrews of multiple species in widely separated geographic regions, data are unavailable about the genetic diversity and phylogeography of Thottapalayam virus (TPMV), a hantavirus originally isolated from an Asian house shrew (Suncus murinus) captured in southern India more than four decades ago. To bridge this knowledge gap, the S, M, and L segments of hantavirus RNA were amplified by reverse transcription polymerase chain reaction from archival lung tissues of Asian house shrews captured in Nepal from January to September 1996. Pair-wise alignment and comparison revealed approximately 80% nucleotide and > 94% amino acid sequence similarity to prototype TPMV. Phylogenetic analyses, generated by maximum likelihood and Bayesian methods, showed geographic-specific clustering of TPMV, similar to that observed for rodent- and soricid-borne hantaviruses. These findings confirm that the Asian house shrew is the natural reservoir of TPMV and suggest a long-standing virus-host relationship.
尽管最近在多个地理区域的不同物种的鼩鼱中发现了遗传上有差异的汉坦病毒，但有关最初从 40 多年前在印度南部捕获的亚洲家鼩（Suncus murinus）中分离出的汉坦病毒（TPMV）的遗传多样性和系统地理学的数据尚不清楚。为了弥补这一知识空白，我们通过逆转录聚合酶链反应从 1996 年 1 月至 9 月在尼泊尔捕获的亚洲家鼩的存档肺组织中扩增了汉坦病毒 RNA 的 S、M 和 L 片段。序列比对和比较显示，其核苷酸和氨基酸序列与原型 TPMV 的相似性分别约为 80%和>94%。最大似然和贝叶斯方法生成的系统发育分析显示，TPMV 存在地理特异性聚类，类似于啮齿动物和鼩鼱传播的汉坦病毒。这些发现证实亚洲家鼩是 TPMV 的天然宿主，并表明病毒与宿主之间存在长期的关系。
