Department of Marine Science, Qinzhou University, 89 Xihuan South Road, Qinzhou 535000, China.
Food Chem Toxicol. 2011 Nov;49(11):2961-7. doi: 10.1016/j.fct.2011.07.044. Epub 2011 Jul 23.
A water-soluble polysaccharide, named as SNP, was extracted and fractioned from the body wall of Sipunculus nudus L. by DEAE-Sepharose anion exchange and Sepharose CL-6B column chromatography. The structural characteristics of SNP investigated by high performance size exclusion chromatography (HP-SEC), Fourier transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS) indicated that SNP was a homogeneous polysaccharide with a molecular mass of 350kD and the monosaccharide composition was determined to be rhamnose (28%), fucose (16%) and galactose (56%). SNP was able to upregulate the expression of cytokines (IL-6 and TNF-α), but did not affect IL-10 secretion by murine macrophages and human peripheral blood mononuclear cells. RT-PCR analysis demonstrated that the SNP also induced the expression of iNOS and COX-2, responsible for the induction of NO and PGE2 respectively, and SNP suppressed the arginase activity. These results suggest that the polysaccharide isolated from S. nudus activates macrophages and has potent immunostimulating activity.
一种水溶性多糖，命名为 SNP，从沙蚕体壁中提取和分离出来。通过 DEAE-Sepharose 阴离子交换和 Sepharose CL-6B 柱层析。通过高效体积排除色谱 (HP-SEC)、傅里叶变换红外光谱 (FT-IR) 和气相色谱-质谱 (GC-MS) 研究 SNP 的结构特征表明，SNP 是一种均一多糖，分子量为 350kD，单糖组成为鼠李糖 (28%)、岩藻糖 (16%) 和半乳糖 (56%)。SNP 能够上调细胞因子 (IL-6 和 TNF-α) 的表达，但不影响小鼠巨噬细胞和人外周血单核细胞中 IL-10 的分泌。RT-PCR 分析表明，SNP 还诱导 iNOS 和 COX-2 的表达，分别负责诱导 NO 和 PGE2，SNP 抑制精氨酸酶活性。这些结果表明，从沙蚕中分离出的多糖激活巨噬细胞，具有很强的免疫刺激活性。
