Li Na, Shen Xianrong, Liu Yuming, Zhang Junling, He Ying, Liu Qiong, Jiang Dingwen, Zong Jie, Li Jiamei, Hou Dengyong, Chen Wei, Wang Qingrong, Luo Qun, Li Kexian
The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
Int J Biol Macromol. 2016 Feb;83:288-96. doi: 10.1016/j.ijbiomac.2015.11.071. Epub 2015 Dec 2.
Sipunculus nudus Linnaeus polysaccharide (SNP) was purified from S. nudus L. via NaOH extraction, trichloroacetic acid deproteination, DEAE-cellulose 52 and Sephacryl S-300 chromatography. The monosaccharide analysis and molecular weight was detected with HPLC. FT-IR, 1H spectrum and 13C NMR spectrum were performed to detect the chemical characteristics. The antioxidant activity was assayed in vitro. The radiation protection effects were detected on mice. The results showed that SNP was composed of mannose, rhamnose, galacturonic acid, glucose, arabinose and fucose, and the average molecular weight was 680 kDa. Above the concentration of 10 mg/mL, SNP showed powerful scavenging activity on hydroxyl radical. In the animals irradiated with a 7.5 Gy γ-rays, the 90 mg/kg and the 270 mg/kg SNP groups survived significantly longer than the radiation control group. In the animals irradiated with a 4.0 Gy γ-rays, SNP showed significant protection effect. The contents of DNA in bone marrow cells were significantly increased by SNP treatment, and the micronucleus rates of 30 mg/kg and 270 mg/kg SNP groups were decrease significantly compared to the radiation control group. These findings suggest that SNP possesses marked antioxidant and bone marrow damage protection capacity which play important roles in the prevention of radiation damage.
通过氢氧化钠提取、三氯乙酸脱蛋白、DEAE-纤维素52和Sephacryl S-300柱色谱法从光裸星虫中纯化得到光裸星虫多糖（SNP）。采用高效液相色谱法检测单糖组成和分子量。通过傅里叶变换红外光谱（FT-IR）、氢谱（1H谱）和碳谱（13C NMR谱）检测其化学特性。体外测定其抗氧化活性。检测其对小鼠的辐射防护作用。结果表明，SNP由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、阿拉伯糖和岩藻糖组成，平均分子量为680 kDa。浓度高于10 mg/mL时，SNP对羟自由基具有较强的清除活性。在接受7.5 Gy γ射线照射的动物中，90 mg/kg和270 mg/kg SNP组的存活时间明显长于辐射对照组。在接受4.0 Gy γ射线照射的动物中，SNP显示出显著的防护作用。SNP处理可显著提高骨髓细胞中的DNA含量，与辐射对照组相比，30 mg/kg和270 mg/kg SNP组的微核率显著降低。这些结果表明，SNP具有显著的抗氧化和骨髓损伤保护能力，在预防辐射损伤中发挥重要作用。
