Department of Endocrinology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
Reprod Biol. 2011 Jul;11(2):117-34. doi: 10.1016/s1642-431x(12)60049-7.
The purpose of the study was to examine the effect of luteal macrophage conditioned medium (LMCM) on progesterone and estradiol production by cultured granulosa cells. Porcine granulosa cells were cultured for 48 h with or without LMCM in the absence or presence of 100 ng/ml LH, FSH or prolactin. Progesterone and estradiol concentrations were measured by radioimmunoassay. Granulosa cells were analyzed histochemically and immunocytochemically for the activity and presence of Δ5, 3β-hydroxysteroid dehydrogenase (3β-HSD), respectively. LMCM stimulated basal and LH-, FSH- or prolactin-induced progesterone secretion. Similarly, LMCM augmented basal and stimulated activity of 3β-HSD in the examined cells. In contrast, LMCM decreased LH- and prolactin-induced estradiol secretion but increased FSH-induced estradiol secretion. These data demonstrate the clear stimulatory effect of LMCM on granulosal progesterone production. It is concluded that substances secreted by macrophages modulate gonadotropin effect on follicular progesterone secretion in a paracrine manner via 3β-HSD activity.
本研究旨在探讨黄体巨噬细胞条件培养基（LMCM）对培养的颗粒细胞孕激素和雌二醇产生的影响。将猪颗粒细胞在有无 100ng/ml LH、FSH 或催乳素的情况下培养 48 小时，有无 LMCM。用放射免疫分析法测定孕激素和雌二醇浓度。用组织化学和免疫细胞化学法分别分析颗粒细胞中 Δ5、3β-羟甾脱氢酶（3β-HSD）的活性和存在情况。LMCM 刺激基础和 LH、FSH 或催乳素诱导的孕激素分泌。同样，LMCM 增强了检查细胞中基础和刺激的 3β-HSD 活性。相比之下，LMCM 减少了 LH 和催乳素诱导的雌二醇分泌，但增加了 FSH 诱导的雌二醇分泌。这些数据表明 LMCM 对颗粒孕激素产生有明显的刺激作用。结论是，巨噬细胞分泌的物质通过 3β-HSD 活性以旁分泌方式调节促性腺激素对卵泡孕激素分泌的作用。
