Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politècnica de Catalunya, Terrassa, Spain.
Biotechnol J. 2011 Oct;6(10):1208-18. doi: 10.1002/biot.201100194. Epub 2011 Aug 29.
Collagen sponges loaded with polyphenols from Hamamelis virginiana were investigated as active materials for chronic wound dressings, evaluating in vitro the inhibition of two major enzymes that impair the wound healing process - myeloperoxidase (MPO) and collagenase. Prior to polyphenols loading, collagen was cross-linked with genipin to improve its biostability. The effect of genipin cross-linking and polyphenol concentration in the development of mechanically and enzymatically stable sponges was studied. The tensile strength of the cross-linked collagen increased with the increase of the cross-linking degree, coupled to decrease in the elongation and the swelling capacity of the sponges. The stability of the sponges to collagenase digestion reached maximum when 1 mM genipin was used. However, the biostability decreased more than 10-fold after loading the sponges with polyphenols (0.5 mg/mL), nevertheless, this effect was partially overcome using higher concentration of polyphenols (1 and 2 mg/mL) to inhibit collagenase. Moreover, the polyphenols released from the sponges were sufficient for complete inhibition of MPO activity. No considerable cytotoxicity of the genipin cross-linked collagen loaded with polyphenols was observed evaluating the NIH 3T3 fibroblasts viability.
载有金缕梅多酚的胶原海绵被研究为用于慢性伤口敷料的活性材料，评估了两种主要酶 - 髓过氧化物酶（MPO）和胶原酶对伤口愈合过程的抑制作用。在多酚负载之前，用京尼平对胶原进行交联以提高其生物稳定性。研究了京尼平交联和多酚浓度对机械和酶稳定海绵的发展的影响。随着交联度的增加，交联胶原的拉伸强度增加，同时海绵的伸长率和溶胀能力降低。当使用 1mM 京尼平时，交联海绵对胶原酶消化的稳定性达到最大值。然而，负载多酚（0.5mg/mL）后，生物稳定性降低了 10 多倍，但是，使用更高浓度的多酚（1mg/mL 和 2mg/mL）来抑制胶原酶可以部分克服这种影响。此外，从海绵中释放的多酚足以完全抑制 MPO 活性。评价 NIH 3T3 成纤维细胞活力时，未观察到载有多酚的京尼平交联胶原的明显细胞毒性。
