Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politècnica de Catalunya, Passeig Ernest Lluch/Rambla Sant Nebridi s/n, 08222 Terrassa, Spain.
Carbohydr Polym. 2013 Feb 15;92(2):989-96. doi: 10.1016/j.carbpol.2012.10.045. Epub 2012 Oct 26.
Laccase-assisted simultaneous cross-linking and functionalization of chitosan/gelatin blends with phenolic compounds from Hamamelis virginiana was investigated for the development of bioactive hydrogel dressings. The potential of these hydrogels for chronic wound treatment was evaluated in vitro, assessing their antibacterial and inhibitory effect on myeloperoxidase and collagenase. Rheological studies revealed that the mechanical properties of the hydrogels were a function of the enzymatic reaction time. Stable hydrogels and resistant to lysozyme degradation were achieved after 2 h laccase reaction. The inhibitory capacity of the hydrogel for myeloperoxidase and collagenase was 32% and 79% respectively after 24 h incubation. Collagenase activity was additionally suppressed by adsorption (20%) of the enzyme onto the hydrogel. Therefore, the bioactive properties of the hydrogels were due to the effect of both released phenolic compounds and the permanently functionalized platform itself. The hydrogels showed antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus.
用金缕梅中的酚类化合物辅助漆酶同时交联和功能化壳聚糖/明胶共混物，用于开发具有生物活性的水凝胶敷料。在体外评估了这些水凝胶治疗慢性伤口的潜力，评估了它们的抗菌和对髓过氧化物酶和胶原酶的抑制作用。流变学研究表明，水凝胶的机械性能是酶反应时间的函数。经过 2 小时漆酶反应，可获得稳定的水凝胶，且不易被溶菌酶降解。水凝胶对髓过氧化物酶和胶原酶的抑制率分别为 24 小时孵育后的 32%和 79%。酶吸附（20%）到水凝胶上，进一步抑制了胶原酶的活性。因此，水凝胶的生物活性特性归因于释放的酚类化合物和永久性功能化平台本身的双重作用。水凝胶对铜绿假单胞菌和金黄色葡萄球菌表现出抗菌活性。
