Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, USA.
Curr Opin Struct Biol. 2011 Aug;21(4):517-22. doi: 10.1016/j.sbi.2011.07.001. Epub 2011 Jul 30.
Structural genomics approaches on integral membrane proteins have been postulated for over a decade, yet specific efforts are lagging years behind their soluble counterparts. Indeed, high throughput methodologies for production and characterization of prokaryotic integral membrane proteins are only now emerging, while large-scale efforts for eukaryotic ones are still in their infancy. Presented here is a review of recent literature on actively ongoing structural genomics of membrane protein initiatives, with a focus on those aimed at implementing interesting techniques aimed at increasing our rate of success for this class of macromolecules.
结构基因组学方法已经被提出用于研究完整膜蛋白超过十年了，但具体的研究进展却远远落后于可溶性蛋白。事实上，高通量生产和鉴定原核完整膜蛋白的方法才刚刚出现，而大规模研究真核完整膜蛋白的工作仍处于起步阶段。本文综述了近期膜蛋白结构基因组学研究的文献，重点介绍了那些旨在实施有趣技术以提高此类大分子成功解析率的研究。
