Department of Microbiology, University of Barcelona, Diagonal 645, Annex, Floor 0, E-08028 Barcelona, Spain.
Antimicrob Agents Chemother. 2011 Oct;55(10):4908-11. doi: 10.1128/AAC.00535-11. Epub 2011 Aug 1.
This study evaluates the occurrence of bacteriophages carrying antibiotic resistance genes in animal environments. bla(TEM), bla(CTX-M) (clusters 1 and 9), and mecA were quantified by quantitative PCR in 71 phage DNA samples from pigs, poultry, and cattle fecal wastes. Densities of 3 to 4 log(10) gene copies (GC) of bla(TEM), 2 to 3 log(10) GC of bla(CTX-M), and 1 to 3 log(10) GC of mecA per milliliter or gram of sample were detected, suggesting that bacteriophages can be environmental vectors for the horizontal transfer of antibiotic resistance genes.
本研究评估了动物环境中携带抗生素耐药基因的噬菌体的发生情况。通过定量 PCR 检测了来自猪、家禽和牛粪便废物的 71 个噬菌体 DNA 样本中的 bla(TEM)、bla(CTX-M)（簇 1 和 9）和 mecA。检测到 bla(TEM)的基因拷贝数 (GC)为 3 到 4 个对数，bla(CTX-M)的 GC 为 2 到 3 个对数，mecA 的 GC 为 1 到 3 个对数，表明噬菌体可以作为抗生素耐药基因水平转移的环境载体。
