紫穗槐叶（豆科）中多酚的测定及自由基清除活性

Determination of polyphenols and free radical scavenging activity of Tephrosia purpurea linn leaves (Leguminosae).

作者信息

Patel Avani, Patel Amit, Patel Amit, Patel N M

机构信息

Department of Pharmacognosy and Phytochemistry, Shri B.M. Shah college of Pharmaceutical Education and Research, Modasa-383 315, North Gujarat, affiliated to Hemchandracharya North Gujarat University, Patan, North Gujarat, India.

出版信息

Pharmacognosy Res. 2010 May;2(3):152-8. doi: 10.4103/0974-8490.65509.

DOI:10.4103/0974-8490.65509

PMID:21808558

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3141306/

Abstract

BACKGROUND

Leaves of Tephrosia purpurea Linn. (sarpankh), belonging to the family Leguminaceae, are used for the treatment of jaundice and are also claimed to be effective in many other diseases. This research work was undertaken to investigate the in vitro antioxidant activity of aqueous and ethanolic extracts of the leaves.

METHOD

The therapeutic effects of tannins and flavonoids can be largely attributed to their antioxidant properties. So, the quantitative determinations were undertaken. All the methods are based on UV-spectrophotometric determination.

RESULT

The total phenolic content of aqueous and ethanolic extracts showed the content values of 9.44 ± 0.22% w/w and 18.44 ± 0.13% w/w, respectively, and total flavonoid estimation of aqueous and ethanolic extracts showed the content values of 0.91 ± 0.08% w/w and 1.56 ± 0.12%w/w, respectively, for quercetin and 1.85 ± 0.08% w/w and 2.54 ± 0.12% w/w, respectively, for rutin. Further investigations were carried out for in vitro antioxidant activity and radical scavenging activity by calculating its percentage inhibition by means of IC(50)values, all the extracts' concentrations were adjusted to fall under the linearity range and here many reference standards like tannic acid, gallic acid, quercetin, ascorbic acid were taken for the method suitability.

CONCLUSION

The results revealed that leaves of this plant have antioxidant potential. The results also show the ethanolic extract to be more potent than the aqueous decoction which is claimed traditionally. In conclusion, T. purpurea Linn. (Leguminosae) leaves possess the antioxidant substance which may be responsible for the treatment of jaundice and other oxidative stress-related diseases.

摘要

背景

紫穗槐叶属于豆科，用于治疗黄疸，据称对许多其他疾病也有效。本研究旨在调查紫穗槐叶水提取物和乙醇提取物的体外抗氧化活性。

方法

单宁和黄酮类化合物的治疗作用很大程度上归因于它们的抗氧化特性。因此，进行了定量测定。所有方法均基于紫外分光光度法测定。

结果

水提取物和乙醇提取物的总酚含量分别为9.44±0.22%w/w和18.44±0.13%w/w，水提取物和乙醇提取物的总黄酮含量，槲皮素分别为0.91±0.08%w/w和1.56±0.12%w/w，芦丁分别为1.85±0.08%w/w和2.54±0.12%w/w。通过计算IC(50)值的抑制百分比，对体外抗氧化活性和自由基清除活性进行了进一步研究，所有提取物的浓度均调整到线性范围内，本方法适用性采用了单宁酸、没食子酸、槲皮素、抗坏血酸等多种参考标准。