Yuan Jian-long, Pu Yi-qiong, Zhang Tong, Tao Jian-sheng, Wu Tao, Lu Hong-guo
Shanghai University of Traditional Chinese Medicine,Shanghai 201203, China.
Zhong Yao Cai. 2011 Jan;34(1):126-9.
In order to obtain the optimal conditions for separating the alkaloids from the extract of Stemona japonica by selecting appropriate cation exchange resins.
Seven types of cation exchange resins were evaluated in separating efficiency with measuring the adsorption ratio and eluting ratio of total alkaloids as indices, and the content of total alkaloids from Stemona japonica was determined as an index by spectrophotometry to choose the optimal technological parameters.
The optimal result of extraction was obtained as Stemona japonica shattered into thick powder, adding eight times amount of 90% alcohol and refluxing and extracting for 3 h (totally extracting for 3 times). Each gram of D004 cation exchange resin could absorb 0.5003 mg of the total alkaloid, and the desorption ratio was 68.45%. The transfer rate of total alkaloids was 58.70%. the product purity of alkaloids was up to 70%.
The D004 cation exchange resin can be used for purificating total alkaloids from Stemona japonica and the established procedure is simple and feasible.
通过选择合适的阳离子交换树脂，获得从百部提取物中分离生物碱的最佳条件。
以7种阳离子交换树脂为研究对象，以总生物碱的吸附率和洗脱率为指标评价其分离效果，采用分光光度法测定百部总生物碱含量，以此为指标筛选最佳工艺参数。
百部粉碎成粗粉，加入8倍量90%乙醇回流提取3 h（共提取3次）时提取效果最佳。每克D004阳离子交换树脂可吸附总生物碱0.5003 mg，解吸率为68.45%，总生物碱转移率为58.70%，生物碱产品纯度达70%。
D004阳离子交换树脂可用于百部总生物碱的纯化，所建立的方法简便可行。
