Ning Xiaoqing, Li Yaohua, Tan Yuanfeng, Chen Yong, Huang Yan, Pan Fuqi
Guangxi College of Traditional Chinese Medicine, Nanning 530001, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jun;36(12):1623-5.
To develop an HPLC method for content determination of protocatechuic acid, chlorogenic acid and caffeic acid in Zhuang medicine Blumea megacephala (Randeria), and explore the content variation of the 3 components of the herbs harvesting in different months, and provide the scientific basis of reasonable application.
The determination was carried out on a Shimadzu VP-ODS column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.1% phosphoric acid in a linear gradient elution. The flow rate was 1 mL min(-1), and the detected wavelength was set at 258, 327nm.
The peak areas and the concentrations of the three components had good linear relationship in the range of 1.7-17 mg x L(-1) for protocatechuic acid, 15.6-156 mg x L(-1) for chlorogenic acid, 3.96-39.6 mg x L(-1) for caffeic acid. The average recoveries were 103.4%, 102.2%, 98.5%, respectively.
The method was proved to be simple, accurate and used for the quality evaluation of Blumea megacephala (Randeria).
建立高效液相色谱法测定壮药大头艾纳香中原儿茶酸、绿原酸和咖啡酸的含量，探讨不同月份采收的药材中这3种成分的含量变化情况，为合理用药提供科学依据。
采用岛津VP-ODS柱（4.6 mm×250 mm，5μm），以乙腈和含0.1%磷酸的水为流动相进行线性梯度洗脱。流速为1 mL·min⁻¹，检测波长设定为258、327 nm。
原儿茶酸在1.7 - 17 mg·L⁻¹、绿原酸在15.6 - 156 mg·L⁻¹、咖啡酸在3.96 - 39.6 mg·L⁻¹范围内，3种成分的峰面积与浓度呈良好的线性关系。平均回收率分别为103.4%、102.2%、98.5%。
该方法简便、准确，可用于大头艾纳香的质量评价。
