Dai Han-yuan, Cao Ke-jian, Zhao An-bin, Chen Xiao-yin
The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China.
Zhong Yao Cai. 2011 Feb;34(2):250-3.
To observe the effects of tonifying kidney,tonifying spleen,invigorating the circulation of blood on the expression of hematopooietic cytokines of bone marrow suppression induced by chemotherapy.
Automated blood analyzer was used to detect the level of RBC and HGB, 14th and 28 days, while real time quantitative RT-PCR was used to detect EPO, EPOR mRNA expression.
Liuwei Dihuang Tang, Buzhong Yiqi Tang and Compound Danshen Decoction group could increase the level of RBC and HGB significantly. Liuwei Dihuang Tang and Buzhong Yiqi Tang groups could increase the mRNA expression level of EPO and EPOR significantly. However, there was no significantly difference when Compound Danshen Decoction group compared with control group on EPO, EPOR mRNA expression level.
The tonifying kidney, tonifying spleen, invigorate the circulation of blood are stable and reliable as to enhance the role of peripheral blood; tonifying kidney, tonifying spleen can improve EPO, EPOR mRNA expression levels, and promote the proliferation of bone marrow hematopoietic stem cells, and promote the differentiation of erythroid blood cells to increase red blood cell line; And invigorate the circulation of blood promote hematopoietic mechanisms have to be further studied.
观察补肾、补脾、活血对化疗所致骨髓抑制造血细胞因子表达的影响。
于第14天和第28天用自动血液分析仪检测红细胞（RBC）和血红蛋白（HGB）水平，采用实时定量逆转录-聚合酶链反应（RT-PCR）检测促红细胞生成素（EPO）、促红细胞生成素受体（EPOR）mRNA表达。
六味地黄丸组、补中益气汤组和复方丹参汤组可显著提高RBC和HGB水平。六味地黄丸组和补中益气汤组可显著提高EPO和EPOR mRNA表达水平。然而，复方丹参汤组与对照组相比，EPO、EPOR mRNA表达水平差异无统计学意义。
补肾、补脾、活血对外周血的提升作用稳定可靠；补肾、补脾可提高EPO、EPOR mRNA表达水平，促进骨髓造血干细胞的增殖，促进红系血细胞的分化，增加红细胞系；而活血促进造血的机制有待进一步研究。
